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Bulletin of Experimental Biology and Medicine

, Volume 154, Issue 1, pp 23–26 | Cite as

Functional Activity of Neutrophils in Diabetes Mellitus and Coronary Heart Disease: Role of Myeloperoxidase in the Development of Oxidative Stress

  • I. V. GorudkoEmail author
  • V. A. Kostevich
  • A. V. Sokolov
  • E. V. Shamova
  • I. V. Buko
  • E. E. Konstantinova
  • V. B. Vasiliev
  • S. N. Cherenkevich
  • O. M. Panasenko
General Pathology and Pathophysiology

We performed a comparative analysis of functional activity of neutrophils in patients with type 2 diabetes mellitus with and without symptoms of CHD. Enhanced H2O2 production by neutrophils in response to N-formyl-Met-Leu-Phe (fMLP) was found in patients with type 2 diabetes mellitus. In patients with type 2 diabetes mellitus associated with CHD, fML-Pinduced release of myeloperoxidase from azurophilic granules of neutrophils was reduced and plasma myeloperoxidase level was elevated. Increased peroxidase activity of myeloperoxidase, reduced plasma catalase activity, and increased levels of TBA-reactive lipid peroxidation products and oxidized glutathione were detected in patients of both groups. Since myeloperoxidase is an important neutrophilic mediator of oxidative stress, its increased activity in the blood can be an additional marker of oxidative stress and cardiovascular risk in patients with diabetes mellitus.

Key Words

myeloperoxidase diabetes mellitus neutrophils oxidative stress coronary heart disease 

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Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • I. V. Gorudko
    • 1
    Email author
  • V. A. Kostevich
    • 2
    • 3
  • A. V. Sokolov
    • 2
    • 3
  • E. V. Shamova
    • 1
  • I. V. Buko
    • 4
  • E. E. Konstantinova
    • 4
  • V. B. Vasiliev
    • 2
  • S. N. Cherenkevich
    • 1
  • O. M. Panasenko
    • 3
  1. 1.Belarusian State UniversityMinskRepablic of Belarussia
  2. 2.Institute of Experimental MedicineNorthwest Department of the Russian Academy of Medical SciencesSt. PetersburgRussia
  3. 3.Institute of Physicochemical MedicineFederal Medico-Biological AgencyMoscowRussia
  4. 4.Republican Scientific and Practical Center “Kardiologiya”MinskRepublic of Belarussia

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