We studied the involvement of DNA synthesis into molecular mechanisms of long-term memory. Nucleoside analogue 5-bromo-2’-deoxyuridine (BrdU) is known to incorporate into synthesizing DNA and prevent subsequent DNA replication from this region. To investigate the effect of BrdU administration on long-term memory, terrestrial gastropods edible snails Helix lucorum were trained in the food aversion paradigm. Single-session training (carrot presentation combined with application of 10% quinine solution, three carrot presentations with 10-min intervals) resulted in the formation of long-term memory that persisted for at least 45 days. BrdU administration (250 mg/kg) 30 min before training impaired long-term memory tested 24 h later. Immunohistochemical study revealed BrdU incorporation in the nuclei of identified neurons of defensive behavior.
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References
P. M. Balaban and I. S. Zakharov, Learning and Development: Common Basis of Two Phenomena [In Russian], Moscow (1992).
N. V. Komissarova, A. A. Tiunova, and K. V. Anokhin, Zh. Vyssh. Nerv. Deyat., 58, No. 6, 700–710 (2008).
K. A. Radyushkin and K. V. Anokhin, Ros. Fiziol. Zh., 83, Nos. 1–2, 11–18 (1997).
K. V. Anokhin, A. A. Tiunova, and S. P. Rose, Eur. J. Neurosci., 15, No. 11, 1759–1765 (2002).
C. H. Bailey and E. R. Kandel, Prog. Brain Res., 169, 179–198 (2008).
H. P. Davis and L. R. Squire, Psychol. Bull., 96, No. 3, 518–559 (1984).
P. W. Frankland, B. Bontempi, L. E. Talton, et al., Science, 304, 881-883 (2004).
L. R. Squire, Learn. Mem., 13, No. 5, 522-529 (2006).
J. Wang, K. Ren, J. Perez, et al., Learn. Mem., 10, No. 6, 503–509 (2003).
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 153, No. 5, pp. 731-734, May, 2012
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Efimova, O.I., Anokhin, K.V. 5-Bromo-2’-Deoxyuridine Impairs Long-Term Food Aversion Memory in Edible Snail. Bull Exp Biol Med 153, 767–770 (2012). https://doi.org/10.1007/s10517-012-1822-5
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DOI: https://doi.org/10.1007/s10517-012-1822-5