We compared the effects of electrical stimulation of primary dissociated neuronal cultures cultured on microelectrode arrays in terms on the expression of c-fos transcriptional factor that is involved in plastic reorganization in neurons. Dissociated hippocampal neurons cultured on multielectrode arrays were exposed to two stimulation protocols: high-frequency and low-frequency stimulations. Expression of c-fos was evaluated using immunofluorescence. Both high-frequency and low-frequency stimulations significantly increased c-fos expression in comparison with non-stimulated control. These findings indicate that c-fos expression can be induced in neuronal cell culture by different types of electrical stimulations and can be used for studying plasticity processes in microphysiological in vitro systems.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 153, No. 5, pp. 538-541, May, 2012
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Sukhanova, A.L., Mineyeva, O.A., Kiselev, I.I. et al. Detection of Trace Processes in the Networks of Neurons Cultured on Microelectrode Arrays. Bull Exp Biol Med 153, 594–597 (2012). https://doi.org/10.1007/s10517-012-1774-9
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DOI: https://doi.org/10.1007/s10517-012-1774-9