Skip to main content
SpringerLink
Log in

Cloning and Expression of Brain-Specific Anion Transporter BSAT1 and Isolation of Monoclonal Antibodies to Its Extracellular Fragment

  • Immunology and Microbiology
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

Brain-specifi c anion transporter BSAT1 extracellular fragment (451-557) cDNA was cloned in a vector for prokaryotic expression, a producer E. coli strain was obtained, and recombinant extracellular fragment BSAT1451-557 was purifi ed and used for immunization of BALB/c mice. Splenic cells from mice with verifi ed immune response were used for hybridoma generation. Several hybridoma clones producing monoclonal antibodies to BSAT1 extracellular fragment were selected. Antibody specifi city was confi rmed by ELISA, immunoblotting with recombinant BSAT1451-557, and immunofl uorescent BSAT1 assay on rat brain sections and cultured HEK293 cells. It was demonstrated that the obtained antibodies specifi cally bind native rat and human BSAT1 and can be used in both fundamental studies of structures forming the blood-brain barrier and development of targeted transport of diagnostic preparations and drugs across the blood-brain barrier.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. V. P. Ch ekhonin, O. I. Gurina, and T. B. Dmitrieva, Monoclonal Antibodies to Neurospecifi c Proteins [in Russian], Moscow (2007).

  2. F. D. Ca massei, R. Boldrini, A. Jenkner, et al., Pediatr. Dev. Pathol., 6, No. 6, 511-519 (2003).

    Article  PubMed  CAS  Google Scholar 

  3. V. P. Ch ekhonin, O. I. Gurina, T. B. Dmitrieva, et al., Byull. Exp. Biol. Med., 132, No. 2, 772-775 (2001).

    Article  CAS  Google Scholar 

  4. R. I. Dm itriev, T. V. Korneenko, A. A. Bessonov, et al., Biochem. Biophys. Res. Commun., 355, No. 4, 1051-1057 (2007).

    Article  PubMed  CAS  Google Scholar 

  5. M. V. Eg orov, A. Tigerström, N. B. Pestov, et al., Protein Expr. Purif., 36, No. 1, 31-39 (2004).

    Article  PubMed  CAS  Google Scholar 

  6. G. Köhler and C. Milstein, Nature, 256, 495-497 (1975).

    Article  PubMed  Google Scholar 

  7. J. Y. Li, R. J. Boado, and W. M. Pardridge, J. Cereb. Blood Flow Metab., 21, No. 1, 61-68 (2001).

    Article  PubMed  CAS  Google Scholar 

  8. N. B. Pestov and J. Rydström, Nat. Protoc., 2, No. 1, 198-202 (2007).

    Article  PubMed  CAS  Google Scholar 

  9. F. Pizzagal li, B. Hagenbuch, B. Stieger, et al., Mol. Microbiol., 16, No. 10, 2283-2296 (2002).

    CAS  Google Scholar 

  10. K. Terpe, Appl. Microbiol. Biotechnol., 60, No. 5, 523-533 (2003).

    PubMed  CAS  Google Scholar 

  11. D. Sugiyama , H. Kusuhara, H. Taniguchi, et al., J. Biol. Chem., 278, No. 44, 43,489-43,495 (2003).

    Article  CAS  Google Scholar 

  12. T. Suzuki, T. Onogawa, N. Asano, et al., Mol. Microbiol., 17, No. 7, 1203-1215 (2003).

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Fundamental and Applied Neurobiology, V. P. Serbskii State Research Center of Social and Forensic Psychiatry, Moscow, Russia

    V. P. Baklaushev, K. Sh. Kardashova, O. I. Gurina, A. V. Semenova, I. A. Ryabukhin & V. P. Chekhonin

  2. Research Center Medical Nanobiotechnologies, Russian State Medical University, Moscow, Russia

    V. P. Baklaushev, A. V. Leopol’d & V. P. Chekhonin

Authors
  1. V. P. Baklaushev
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. Sh. Kardashova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. O. I. Gurina
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. V. Leopol’d
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. V. Semenova
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. I. A. Ryabukhin
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. V. P. Chekhonin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to V. P. Baklaushev.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 152, No. 12, pp. 678-683, December, 2011

Rights and permissions

Reprints and permissions

About this article

Cite this article

Baklaushev, V.P., Kardashova, K.S., Gurina, O.I. et al. Cloning and Expression of Brain-Specific Anion Transporter BSAT1 and Isolation of Monoclonal Antibodies to Its Extracellular Fragment. Bull Exp Biol Med 152, 734–738 (2012). https://doi.org/10.1007/s10517-012-1619-6

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10517-012-1619-6

Keywords

Search

Navigation