cDNA fragment encoding neuron-specific enolase was amplified from the cDNA library of human brain. Then the fragment was cloned for expression in E. coli using the vector pET28-a. High level of neuron-specific enolase expression was confirmed by SDS-PAAG electrophoresis and immunochemical identity by immunoblot analysis. The constructed producer strain is the cheapest source of neuron-specific enolase suitable for the use in diagnostic applications.
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References
V. P. Chekhonin, O. I. Gurina, and T. B. Dmitrieva, Monoclonal Antibodies to Neurospecifi c Proteins [in Russian], Moscow (2007).
S. T. Brady and R. J. Lasek, Cell, 23, No. 2, 515-523 (1981).
A. Chiaretti, G. Barone, R. Riccardi, et al., Neurology, 72, No. 7, 609-616 (2009).
K. Kato, R. Asai, A. Shimizu, et al., Clin. Chim. Acta, 127, No. 3, 353-363 (1983).
K. A. Pavlov, O. I. Gurina, T. B. Dmitrieva, et al., Bull. Exp. Biol. Med., 146, No. 5, 599-604 (2008).
J. Sambrook and D . W. Russel, Molecular Cloning: a Laboratory Manual, N.Y. (2001).
K. Shigekawa and W. J. Dower, Biotechniques, 6, No. 8, 742-751 (1988).
A. A. Topjian, R. Lin R, M. C. Morris, et al., Pediatr. Crit. Care Med., 10, No. 4, 479-490 (2009).
E. Van Obberghen, J. Kamholz, J. G. Bishop 3rd, et al., Neurosci. Res., 19, No. 4, 450-456 (1988).
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Translated from Byulleten’ Eksperimental”noi Biologii i Meditsiny, Vol. 152, No. 8, pp. 166-169, August, 2011
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Pavlov, K.A., Gurina, O.I., Antonova, O.M. et al. Cloning and Expression of Human Neuron-Specific Enolase cDNA in Escherichia coli . Bull Exp Biol Med 152, 206–209 (2011). https://doi.org/10.1007/s10517-011-1489-3
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DOI: https://doi.org/10.1007/s10517-011-1489-3