Genotypic variability of αENaC mRNA in adult rat kidney caused by deletion at the 3’-end of α-subunit DNA was studied by real-time PCR using specifi c probes with a fl uorescent dye (TaqMan). It was found that mRNA deletion forms (a and b), products of alternative splicing of aENaC gene, are absent in the cortex of adult rat kidney.
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K. Banasikowska, M. Post, and E. Cutz, et. al., Am. J. Physiol. Lung Cell. Mol. Physiol., 287, No. 3, L608-L615 (2004).
S. Chu, C. A. Cockrell, and T. J. Ferro, Biochem. Biophys. Res. Commun., 303, No. 4, 1159–1168 (2003).
H. Garty and L.G. Palmer, Physiol. Rev., 77, No. 2, 359–396 (1997).
X. J. Li, R. H. Xu, W. B. Guggino, and S. H. Snyder, Mol. Pharmacol., 47, No. 6, 1133–1140 (1995).
G. Otulakowski, T. Freywald, Y. Wen, and H. O’Brodovich, Am. J. Physiol. Lung Cell. Mol. Physiol., 281, No. 5, L1219-L1231 (2001).
M. F. Shehata, Int. Arch. Med., 2, No. 1, 5 (2009)
M. F. Shehata, Int. Arch. Med., 2, No. 1, 28 (2009).
C. P. Thomas, N. A. Doggett, R. Fisher, and J. B. Stokes, J. Biol. Chem., 271, No. 42, 26,062–26,066 (1996).
C. P. Thomas, S. Auerbach, J. B. Stokes, and K. A. Volk, Am. J. Physiol., 274, No. 5, Pt. 1, C1312-C1323 (1998).
H. Xu and S. Chu, Am. J. Physiol. Lung Cell. Mol. Physiol., 293, No. 6, L1454–L146 2 (2007).
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 151, No. 6, pp. 655–658, June, 2011
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Logvinenko, N.S., Herbeck, Y.E. & Ivanova, L.N. Investigation on Genetic Heterogeneity of α-Subunit of the Epithelial Sodium Channel in Rat Renal Cortex. Bull Exp Biol Med 151, 709–712 (2011). https://doi.org/10.1007/s10517-011-1422-9
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DOI: https://doi.org/10.1007/s10517-011-1422-9