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Simple Method of Specimen Preparation for Scanning Electron Microscopy

Bulletin of Experimental Biology and Medicine volume 151, pages 378–382 (2011)Cite this article

We compared conventional method for specimen (cell cultures, tissue specimens) preparation for scanning electron microscopy and a method without sputtering and critical-point drying. OTO-method (osmium-thiocarbohydrazide-osmium) with sample impregnation with hexamethyldisilazane followed by air drying was used as an alternative method. Excellent preservation of surface ultrastructures and electrical conductivity was proved. The method is easy to use and does not require additional costs for equipment.

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Affiliations

  1. Department of Fundamental Medicine, M. V. Lomonosov Moscow State University, Moscow, Russia

    S. V. Buravkov & V. P. Chernikov

  2. Institute for Biomedical Problems, Russian Academy of Sciences, Moscow, Russia

    L. B. Buravkova

Authors
  1. S. V. Buravkov
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  2. V. P. Chernikov
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  3. L. B. Buravkova
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Corresponding author

Correspondence to S. V. Buravkov.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 151, No. 3, pp. 356–360, March, 2011

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Buravkov, S.V., Chernikov, V.P. & Buravkova, L.B. Simple Method of Specimen Preparation for Scanning Electron Microscopy. Bull Exp Biol Med 151, 378–382 (2011). https://doi.org/10.1007/s10517-011-1335-7

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  • DOI: https://doi.org/10.1007/s10517-011-1335-7

Key Words

  • scanning electron microscopy
  • OTO-method (osmium-thiocarbohydrazideosmium)
  • specimen preparation
  • cell culture