Dynorphin A (1-17) in concentrations of 10−8–10−9 M inhibits phytohemagglutinin (2.5 μg/ml)-induced proliferative response of mononuclear fraction lymphocytes. In mitogen-stimulated cultures, 10−8 M dynorphin A (1-17) stimulates the production of IL-4, inhibits the production of IL-2, and does not modify the production of IFN-γ. Nonselective κ-receptor antagonist naloxone and selective antagonist binaltorphimine hydrochloride abolish the inhibitory effects of both dynorphin A concentrations on the lymphocyte proliferative response. On the other hand, evaluation of the effect of κ-receptor blockade on the production of IL-2 and IL-4 showed that this effect depends on peptide concentration and antagonist type. Hence, the results attest to an important role of κ-receptors in modulation of functional activity of immune cells.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 150, No. 7, pp. 54–58, July, 2010
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Gein, S.V., Siytchihin, A.A. Effect of Dynorphin A (1-17) on Proliferation and IL-2, IL-4, IFN-γ Production by Peripheral Blood Mononuclears. Bull Exp Biol Med 150, 48–52 (2010). https://doi.org/10.1007/s10517-010-1065-2
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DOI: https://doi.org/10.1007/s10517-010-1065-2