We analyzed restriction fragment length polymorphism of lipL32 gene encoding outer membrane protein in three leprospira genomic species (L. interrogans, L. kirschneri, and L. borgpetersenii) using Bsa29 I and Bam HI restriction endonucleases. It was found that restriction profiles of the studied gene were similar at both the intraspecific and serogroup levels; variability was revealed only at the interspecific level, which can be explained by relatively low variability of lipL32 gene.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 149, No. 5, pp. 543-545, May, 2010
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Vaganova, A.N., Freilikhman, O.A., Stoyanova, N.A. et al. Interspecific Polymorphism of Gene lipL32 Restriction Profiles of Pathogenic Leptospires. Bull Exp Biol Med 149, 609–611 (2010). https://doi.org/10.1007/s10517-010-1005-1
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DOI: https://doi.org/10.1007/s10517-010-1005-1