Skip to main content

Advertisement

Springer Nature Link
Log in

Effect of Interleukin-1β on the Expression of Tight Junction Proteins in the Culture of HaCaT Keratinocytes

  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

We studied the effect of IL-1β on the expression of tight junction proteins (occludin and claudins) in cultured HaCaT keratinocytes and changes of transepithelial resistance. Addition of IL-1β had little effect on transepithelial resistance, increased the expression of claudin-1, and did not modify the expression of occludin. In other tissues, IL-1β also increases claudin-1 expression, but significantly decreases occludin expression. These changes are accompanied by the reduction of transepithelial resistance. The IL-1β-induced increase in the expression of claudin-1 in cultured HaCaT keratinocytes simulates the appearance of claudin-1 at the early stage of skin wound healing. It is accompanied by an increase in IL-1β concentration in the wound fluid.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+
from $39.99 /Month
  • Starting from 10 chapters or articles per month
  • Access and download chapters and articles from more than 300k books and 2,500 journals
  • Cancel anytime
View plans

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Explore related subjects

Discover the latest articles and news from researchers in related subjects, suggested using machine learning.

References

  1. T. Abe, E. Sugano, Y. Saigo, and M. Tamai, Invest. Ophthalmol. Vis. Sci., 44, No. 9, 4097-4104 (2003).

    Article  PubMed  Google Scholar 

  2. R. M. Al-Sadi and T. Y. Ma, J. Immunol., 178, No. 7, 4641-4649 (2007).

    CAS  PubMed  Google Scholar 

  3. A. Arabzadeh, T. C. Troy, and K. Turksen, J. Invest. Dermatol., 128, No. 1, 237-240 (2008).

    CAS  Google Scholar 

  4. J. M. Brandner, S. Kief, C. Grund, et al., Eur. J. Cell. Biol., 81, No. 5, 253-263 (2002).

    Article  CAS  PubMed  Google Scholar 

  5. M. Cumberbatch, R. J. Dearman, and I. Kimber, Adv. Exp. Med. Biol., 417, 125-128 (1997).

    CAS  PubMed  Google Scholar 

  6. T. J. Fahey 3rd, B. Sherry, K. J. Tracey, et al., Cytokine, 2, No. 2, 92-99 (1990).

    Article  CAS  PubMed  Google Scholar 

  7. M. Furuse, M. Hata, K. Furuse, et al., J. Cell. Biol., 156, No. 6, 1099-1111 (2002).

    Article  CAS  PubMed  Google Scholar 

  8. C. Hammerberg, Z. Bata-Csorgo, J. J. Voorhees, and K. D. Cooper, Arch. Dermatol. Res., 290, No. 7, 367-374 (1998).

    Article  CAS  PubMed  Google Scholar 

  9. J. Kobayashi, T. Inai, K. Morita, et al., Cytokine, 28, Nos. 4-5, 186-189 (2004).

    Article  CAS  PubMed  Google Scholar 

  10. N. Maas-Szabowski, H. J. Stark, and N. E. Fusenig, J. Invest. Dermatol., 114, No. 6, 1075-1084 (2000).

    CAS  Google Scholar 

  11. M. Malminen, V. Koivukangas, J. Peltonen, et al., Br. J. Dermatol., 149, No. 2, 255-260 (2003).

    Article  CAS  PubMed  Google Scholar 

  12. B. Tebbe, J. Mankertz, C. Schwarz, et al., Arch. Dermatol. Res., 294, Nos. 1-2, 14-18 (2002).

    Article  CAS  PubMed  Google Scholar 

  13. E. Variouchina, M. Antsiferova, G. Aleksandrov, et al., Rus. J. Immunol., 8, Nos. 2-3, 155-164 (2003).

    Google Scholar 

  14. R. E. Watson, R. Poddar, J. M. Walker, et al., J. Pathol., 212, No. 4, 450-458 (2007).

    Article  CAS  PubMed  Google Scholar 

  15. Y. Yoshida, K. Morita, A. Mizoguchi, et al., Arh. Dermatol. Res., 293, No. 5, 239-244 (2001).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of General Physiology, Biological and Soil Faculty, St. Petersburg State University, St. Petersburg, Russia

    V. L. Rozlomiy & A. G. Markov

Authors
  1. V. L. Rozlomiy
    View author publications

    Search author on:PubMed Google Scholar

  2. A. G. Markov
    View author publications

    Search author on:PubMed Google Scholar

Corresponding author

Correspondence to V. L. Rozlomiy.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 149, No. 3, pp. 251-254, March, 2010

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rozlomiy, V.L., Markov, A.G. Effect of Interleukin-1β on the Expression of Tight Junction Proteins in the Culture of HaCaT Keratinocytes. Bull Exp Biol Med 149, 280–283 (2010). https://doi.org/10.1007/s10517-010-0927-y

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10517-010-0927-y

Key Words