We studied the effect of IL-1β on the expression of tight junction proteins (occludin and claudins) in cultured HaCaT keratinocytes and changes of transepithelial resistance. Addition of IL-1β had little effect on transepithelial resistance, increased the expression of claudin-1, and did not modify the expression of occludin. In other tissues, IL-1β also increases claudin-1 expression, but significantly decreases occludin expression. These changes are accompanied by the reduction of transepithelial resistance. The IL-1β-induced increase in the expression of claudin-1 in cultured HaCaT keratinocytes simulates the appearance of claudin-1 at the early stage of skin wound healing. It is accompanied by an increase in IL-1β concentration in the wound fluid.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 149, No. 3, pp. 251-254, March, 2010
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Rozlomiy, V.L., Markov, A.G. Effect of Interleukin-1β on the Expression of Tight Junction Proteins in the Culture of HaCaT Keratinocytes. Bull Exp Biol Med 149, 280–283 (2010). https://doi.org/10.1007/s10517-010-0927-y
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DOI: https://doi.org/10.1007/s10517-010-0927-y