Skip to main content
SpringerLink
Log in

Preparation and Characterization of Culture of CD146+ Cells from Human Adipose Tissue

  • Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

A method for isolation of homogenous culture of cells expressing CD146 marker from adipose tissue lipoaspirate was developed. The resultant clonogenic cultures retained high proliferative activity, immunophenotype, and morphology after numerous passages. The presence of insulin in the medium served as the selective factor for maintenance of the population phenotype. These cultures effectively differentiate into CD31+ endothelial cells and can be used in regenerative medicine.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. G. Astori, F. Vignati, S. Bardelli, et al., J. Trans. Med., 5, 55 (2007).

    Article  Google Scholar 

  2. D. Baksh, R. Yao, and R. S. Tuan, Stem Cell, 25, No. 6, 1384-1392 (2007).

    Article  CAS  Google Scholar 

  3. N. Bardin, V. Moal, F. Anfosso, et al., Thromb. Haemos., 90, No. 5, 915-920 (2003).

    CAS  Google Scholar 

  4. A. I. Caplan, J. Pathol., 217, No. 2, 318-324 (2009).

    Article  CAS  PubMed  Google Scholar 

  5. M. Cerletti, M. J. Molloy, K. Tomczak, et al., J. Cell Sci., 119, Pt. 15, 3117-3127 (2006).

    Article  CAS  PubMed  Google Scholar 

  6. M. Crisan, S. Yap, L. Casteilla, et al., Cell Stem Cell, 3, No. 3, 301-313 (2008).

    Article  CAS  PubMed  Google Scholar 

  7. B. Delorme, J. Ringe, N. Gallay, et al., Blood, 111, No. 5, 2631-2635 (2008).

    Article  CAS  PubMed  Google Scholar 

  8. L. S. Ferreira, S. Gerecht, H. F. Shieh, et al., Circ. Res., 101, No. 3, 286-294 (2007).

    Article  CAS  PubMed  Google Scholar 

  9. B. Guezguez, P. Vigneron, N. Lamerant, et al., J. Immunol., 179, No. 10, 6673-6685 (2007).

    CAS  PubMed  Google Scholar 

  10. K. Lin, Y. Matsubara, Y. Masuda, et al., Cytotherapy, 10, No. 4, 417-426 (2008).

    Article  CAS  PubMed  Google Scholar 

  11. P. Mancuso, P. Antoniotti, J. Quarna, et al., J. Clin. Cancer Res., 15, No. 1, 267-273 (2009).

    Article  CAS  Google Scholar 

  12. J. B. Mitchell, K. McIntosh, S. Zvonic, et al., Stem Cells, 24, No. 2, 376-385 (2006).

    Article  PubMed  Google Scholar 

  13. D. A. Rider, C. Dombrowski, A. A. Sawyer, et al., Ibid., 26, No. 6, 1598-1608 (2008).

    Article  CAS  PubMed  Google Scholar 

  14. B. Sacchetti, A. Funari, S. Michienzi, et al., Cell, 131, No. 2, 324-336 (2007).

    Article  CAS  PubMed  Google Scholar 

  15. K. E. Schwab, P. Hutchinson, and C. E. Gargett, Human Reprod., 23, No. 4, 934-943 (2008).

    Article  CAS  Google Scholar 

  16. B. M. Seo, M. Miur, S. Gronthos, et al., Lancet, 364, 149-155 (2004).

    Article  CAS  PubMed  Google Scholar 

  17. I. M. Shih, T. L. Wang, and W. H. Westra, Clin. Cancer Res., 2, No. 3, 569-575 (1996).

    CAS  PubMed  Google Scholar 

  18. A. Sorrentino, M. Ferracin, G. Castelli, et al., Exp. Hematol., 36, No. 8, 1035-1046 (2008).

    Article  CAS  PubMed  Google Scholar 

  19. C. Tripodo, A. DiBernardo, M. P. Ternullo, et al., Haematologica, 94, No. 1, 127-130 (2009).

    Article  PubMed  Google Scholar 

  20. H. Zhang, V. Vakil, M. Braunstein, et al., Blood, 105, No. 8, 3286-3294 (2005).

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. ReMeTex Company, Moscow, Russia

    A. A. Rzhaninova, A. V. Kulikov, I. A. Spirova, E. E. Kirienko, A. V. Volkov & D. V. Goldshtein

  2. Medical Genetic Center, Russian Academy of Medical Sciences, Moscow, Russia

    A. A. Rzhaninova, I. A. Spirova, E. E. Kirienko & D. V. Goldshtein

Authors
  1. A. A. Rzhaninova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. V. Kulikov
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. I. A. Spirova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. E. E. Kirienko
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. V. Volkov
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. D. V. Goldshtein
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. A. Rzhaninova.

Additional information

Translated from Kletochnye Tehnologii v Biologii i Medicine, No. 1, pp. 3-9, January, 2010

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rzhaninova, A.A., Kulikov, A.V., Spirova, I.A. et al. Preparation and Characterization of Culture of CD146+ Cells from Human Adipose Tissue. Bull Exp Biol Med 149, 113–118 (2010). https://doi.org/10.1007/s10517-010-0888-1

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10517-010-0888-1

Key Words

Search

Navigation