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Relationship between Suppression of E6 and E7 Virus Oncogenes and Expression of Apoptosis and Cell Cycle Genes in Cervical Cancer Culture

  • Oncology
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Bulletin of Experimental Biology and Medicine Aims and scope

The effects of short interfering RNA suppressing the expression of E6 and E7 human papilloma virus (type 18) on the expression of apoptosis and cell cycle genes were studied in HeLa cells. Changes in the transcription profiles were evaluated using DNA microarray and real-time reverse-transcription PCR. Cell transfection with anti-E6 and anti-E7 short interfering RNA moderately reduced the expression of mRNA for CDC25C, GRB2, GTSE1, and PLK1 genes and induced expression of CDKN1A (p21CIP) gene mRNA. In addition, culture proliferation was inhibited and morphological changes characteristic of differentiation and cell aging developed.

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Authors and Affiliations

  1. Russian State Medical University, Moscow, Russia

    E. V. Khokhlova, A. N. Shkoporov, N. N. Volodin, B. A. Efimov, K. A. Pavlov & L. I. Kafarskaia

Authors
  1. E. V. Khokhlova
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  2. A. N. Shkoporov
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  3. N. N. Volodin
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  4. B. A. Efimov
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  5. K. A. Pavlov
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  6. L. I. Kafarskaia
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Corresponding author

Correspondence to A. N. Shkoporov.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 149, No. 1, pp. 80–84, January, 2010

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Khokhlova, E.V., Shkoporov, A.N., Volodin, N.N. et al. Relationship between Suppression of E6 and E7 Virus Oncogenes and Expression of Apoptosis and Cell Cycle Genes in Cervical Cancer Culture. Bull Exp Biol Med 149, 73–77 (2010). https://doi.org/10.1007/s10517-010-0879-2

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  • DOI: https://doi.org/10.1007/s10517-010-0879-2

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