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  • Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
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Morphological Evaluation of Cytoarchitectonics of Aortic Graft at the Biotechnological Stage with Analysis of Changes in Laser-Induced Fluorescence Spectra

Bulletin of Experimental Biology and Medicine volume 148, pages 684–688 (2009)Cite this article

We performed a fluorescent microscopic examination of human and animal aortic grafts at different stages of decellularization. Treatment of aortic grafts with trypsin/EDTA solution for 48 h leads to their complete decellularization and preserved the connective tissue fiber backbone, which gains a netlike structure. The use of this protocol of decellularization leads to disappearance of subintimal calcium deposits in human aortic grafts. Differences in laserinduced fluorescence spectra of aortas before decellularization and at different stages of this process were revealed. Our findings suggest that the use of fluorescence induced by excimer lasers is promising for identification of the composition of biological tissues, analysis of their state, and the presence of changes.

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Affiliations

  1. E. N. Meshalkin Research Institute of Circulatory Pathology, Federal Agency for High-Technological Medical Care, Novosibirsk, Russia

    D. V. Subbotin, P. M. Larionov, D. S. Sergeevichev, O. A. Subbotina, G. S. Zaitsev & R. B. Novruzov

  2. Institute of Theoretical and Applied Mechanics, Siberian Division of Russian Academy of Sciences, Novosibirsk, Russia

    A. M. Orishich, A. N. Malov, N. A. Maslov & I. A. Rozhin

  3. Research Institute of Regional Pathology and Pathomorphology, Siberian Division of Russian Academy of Medical Sciences, Novosibirsk, Russia

    E. L .Lushnikova & L. M. Nepomnyashih

Authors
  1. D. V. Subbotin
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  2. P. M. Larionov
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  3. D. S. Sergeevichev
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  4. O. A. Subbotina
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  5. G. S. Zaitsev
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  6. R. B. Novruzov
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  7. A. M. Orishich
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  8. A. N. Malov
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  9. N. A. Maslov
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  10. I. A. Rozhin
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  11. E. L .Lushnikova
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  12. L. M. Nepomnyashih
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Corresponding author

Correspondence to P. M. Larionov.

Additional information

Translated from Kletochnye Tehnologii v Biologii i Medicine, No. 4, pp. 191-196, December, 2009

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Subbotin, D.V., Larionov, P.M., Sergeevichev, D.S. et al. Morphological Evaluation of Cytoarchitectonics of Aortic Graft at the Biotechnological Stage with Analysis of Changes in Laser-Induced Fluorescence Spectra. Bull Exp Biol Med 148, 684–688 (2009). https://doi.org/10.1007/s10517-010-0794-6

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  • DOI: https://doi.org/10.1007/s10517-010-0794-6

Key Words

  • human and animal aortic grafts
  • decellularization
  • fluorescent microscopy
  • laser-induced fluorescence
  • excimer laser