Binding and uptake of complexes of endotoxin and low-density lipoproteins (LPS—LDL) in the arterial wall and mononuclear phagocytes were studied under in vitro conditions. Incubation of aortic explants from Wistar rats with complexes of 125I—LDL and S. minnesota R595 LPS or 125I--LDL was accompanied by a 6-fold increase in binding (0°C) and 2-fold increase in the uptake (37°C) of LDL—LPS complexes as compared to free LDL. Binding and degradation of 125I—LDL—LPS complexes in the culture of peritoneal macrophages were higher compared to the corresponding parameters for free 125I—LDL. Our results suggest that the formation of LDL—LPS complexes is followed by the increased binding and accumulation of LDL in the arterial wall and macrophages. These changes probably induce the cascade of major atherogenic events in the vascular wall.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 147, No. 2, pp. 148–151, February, 2009
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Schwartz, Y.S., Dushkin, M.I. In Vitro Accumulation of Complexes of Endotoxin and Low-Density Lipoproteins by Macrophages and Arterial Wall. Bull Exp Biol Med 147, 189–192 (2009). https://doi.org/10.1007/s10517-009-0488-0
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DOI: https://doi.org/10.1007/s10517-009-0488-0