Fluorescence intensity of thioflavin T fluorogenic label increased significantly as a result of formation of enzyme-inhibitory complex with acetylcholinesterase of human blood erythrocytes. Thioflavin T is a reversible inhibitor, selectively reacting with acetylcholinesterase. Thioflavin T fluorescence intensity is proportional to acetylcholinesterase activity for the studied interval of enzyme activities. A rapid fluorescent method for measuring acetylcholinesterase activity is proposed.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 147, No. 1, pp. 118–119 , January, 2009
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Antokhin, A.M., Gainullina, E.T., Ryzhikov, S.B. et al. Rapid Method for Measurement of Acetylcholinesterase Activity. Bull Exp Biol Med 147, 109–110 (2009). https://doi.org/10.1007/s10517-009-0436-z
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DOI: https://doi.org/10.1007/s10517-009-0436-z