A fragment of the nucleotide sequence encoding polypeptide binding to HT-29 epithelial cells was cloned from VMKB44 Bifidobacterium longum genome library using surface phage display technology. Sequencing of this polypeptide consisting of 26 amino acid residues showed that it is an extracellular fragment of a large BL0155 transmembrane protein belonging to the ABC transport protein superfamily. The genes encoding homologues of this protein were detected in genomes of not only bifidobacteria of different species, but also in many other enteric commencals and pathogens.
Similar content being viewed by others
References
M. F. Bernet, D. Brassart, J. R. Neeser, and A. L. Servin, Appl. Environ. Microbiol., 59, No. 12, 4121–4128 (1993).
J. Bjerketorp, A. Rosander, M. Nilsson, et al., J. Med. Microbiol., 53, No. 10, 945–951 (2004).
K. Jacobsson, A. Rosander, J. Bjerketorp, and L. Frykberg, Biol. Proced. Online, 5, 123–135 (2003).
H. Morita, F. He, T. Fuse, et al., Microbiol. Immunol., 46, No. 4, 293–297 (2002).
P. F. Perez, Y. Minnaard, E. A. Disalvo, and G. L. De Antoni, Appl. Environ. Microbiol., 64, No. 1, 21–26 (1998).
M. Stahl, G. Molin, A. Persson, et al., Int. J. Syst. Bacteriol., 40, 189–193 (1990).
T. Wall, S. Roos, K. Jacobsson, et al., Microbiology, 149, No. 12, 3493–3505 (2003).
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 146, No. 12, pp. 682–685, December, 2008
Rights and permissions
About this article
Cite this article
Shkoporov, A.N., Khokhlova, E.V., Kafarskaia, L.I. et al. Search for Protein Adhesin Gene in Bifidobacterium Longum Genome Using Surface Phage Display Technology. Bull Exp Biol Med 146, 782–785 (2008). https://doi.org/10.1007/s10517-009-0423-4
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-009-0423-4