Skip to main content

Lamin B Content in Chromatin Fractions after Purification of Nuclear Matrix from Cells of Different Types

Bulletin of Experimental Biology and Medicine volume 146, pages 580–584 (2008)Cite this article

We analyzed the content of lamin B, one of the main proteins of the nuclear membrane, in different chromatin fractions obtained during purification of the nuclear matrix from different cell types. Depending on cell type and nuclear matrix preparation technique, lamin B was found in different not associated with matrix chromatin compartments. This effect was observed after chromatin extraction with ammonium chloride after nucleolysis and after chromatin extraction with sodium chloride before nuclease treatment. These findings suggest that the structure of the nuclear matrix is destabilized in certain extraction procedures and that studies of subcompartmentalization of nuclear macromolecules require additional control of nuclear matrix integrity.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

43,34 €

Price includes VAT (Finland)
Tax calculation will be finalised during checkout.

References

  1. Ye. V. Sheval, S. Yu. Kurchashova, E. R. Timirbulatova, et al., Tsytologiya, No. 47, 77–82 (2005).

  2. J. L. Broers, F. C. Ramaekers, G. Bonne, et al., Physiol. Rev., 86, No. 3, 967–1008 (2006).

    PubMed  Article  CAS  Google Scholar 

  3. R. Hancock, Biol. Cell., 96, No. 8, 595–601 (2004).

    PubMed  Article  CAS  Google Scholar 

  4. R. Hancock, Chromosoma, 109, No. 4, 219–225 (2000).

    PubMed  Article  CAS  Google Scholar 

  5. D. C. He, J. A. Nickerson, S. Penman, J. Cell Biol., 110, No. 3, 569–580 (1990).

    PubMed  Article  CAS  Google Scholar 

  6. D. A. Jackson, Chromosome Res., 11, No. 5, 387–401 (2003).

    PubMed  Article  CAS  Google Scholar 

  7. M. A. Lapshina, I. I. Parkhomenko, and A. A. Terentiev, An. N.Y. Acad. Sci., 1090, 177–181 (2006).

    Article  CAS  Google Scholar 

  8. H. Ma, A. J. Siegel, and R. Berezney, J. Cell Biol., 146, No. 3, 531–541 (1999).

    PubMed  Article  CAS  Google Scholar 

  9. M. A. Narang, R. Dumas, L. M. Ayer, and R. A. Gravel, Hum. Mol. Genet., 13, No. 1, 15–23 (2004).

    PubMed  Article  CAS  Google Scholar 

  10. J. Nickerson, J. Cell. Sci., 114, Pt. 3, 463–474 (2001).

    PubMed  CAS  Google Scholar 

  11. R. Schirmbeck, A. von der Weth, and W. Deppert, J. Virol., 67, No. 2, 894–901 (1993).

    PubMed  CAS  Google Scholar 

  12. K. M. Tsutsui, K. Sano, and K. Tsutsui, Acta. Med. Okayama., 59, No. 4, 113–120 (2005).

    PubMed  CAS  Google Scholar 

  13. S. Vlcek, T. Dechat, R. Foisner, Cell. Mol. Life Sci., 58, Nos. 12–13, 1758–1765 (2001).

    PubMed  Article  CAS  Google Scholar 

Download references

Author information

Affiliations

  1. Laboratory of Molecular Biology, Institute of Problems of Chemical Physics, Russian Academy of Sciences, Chernogolovka, Russia

    M. A. Lapshina, I. I. Parkhomenko, R. I. Papina & A. A. Terentiev

Authors
  1. M. A. Lapshina
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. I. I. Parkhomenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. I. Papina
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. A. Terentiev
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M. A. Lapshina.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 146, No. 11, pp. 515–519, November, 2008

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Lapshina, M.A., Parkhomenko, I.I., Papina, R.I. et al. Lamin B Content in Chromatin Fractions after Purification of Nuclear Matrix from Cells of Different Types. Bull Exp Biol Med 146, 580–584 (2008). https://doi.org/10.1007/s10517-009-0354-0

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10517-009-0354-0

Key Words

  • lamin B
  • soluble chromatin
  • nuclear matrix
  • purification