Co-localization of Chlamydia trachomatis incorporation membrane proteins with cell organelles was studied in HeLa cell culture after transfection by expressing vectors carrying incA, incB, incC, incD, incE, incF, incG genes, respectively, fused with the marker green fluorescent protein (EGFP) gene. The prokaryotic proteins were co-located with compartments of the secretory pathway of the eukaryotic cell in the course of biogenesis.
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References
E. S. Kostrjukova, F. V. Korobova, V. N. Lazarev, et al., Byull. Eksp. Biol. Med., 139, No. 5, 562–567 (2005).
M. Chalfie, Y. Tu, G. Euskirchen, et al., Science, 263, 802–805 (1994).
K. A. Fields and T. Hackstadt, Annu. Rev. Cell Dev. Biol., 18, 221–245 (2002).
S. S. Grieshaber, N. A. Grieshaber, and T. Hackstadt, J. Cell Sci., 116, Pt. 18, 3793–3802 (2003).
T. Hackstadt, D. D. Rockey, R. A. Heinzen, and M. A. Scidmore, EMBO J., 15, No. 5, 964–977 (1996).
M. Kawasaki, K. Nakayama, and S. Wakatsuki, Curr. Opin. Struct. Biol., 15, No. 6, 681–689 (2005).
J. Lippincott-Schwartz, T. H. Roberts, and K. Hirschberg, Annu. Rev. Cell Dev. Biol., 16, 557–589 (2000).
E. M. Manders, F. J. Verbeek, and J. A. Aten, J. Micros., 169, 375–392 (1993).
A. R. Moorhead, K. A. Rzomp, and M. A. Scidmore, Infect. Immun., 75, No. 2, 781–791 (2007).
J. W. Moulder, Microbiol. Rev., 55, No. 1, 143–190 (1991).
Y. Pannekoek and A. van der Ende, Drugs Today, 42, Suppl. A, 65–73 (2006).
D. D. Rockey, M. A. Scidmore, J. P. Bannantine, and W. J. Brown, Microbes Infect., 4, No. 3, 333–340 (2002).
K. A. Rzomp, A. R. Moorhead, and M. A. Scidmore, Infect. Immun., 74, No. 9, 5362–5373 (2006).
K. A. Rzomp, L. D. Scholtes, B. J. Briggs, et al., Ibid., 71, No. 10, 5855–5870 (2003).
J. Schachter, Chlamydia: Intracellular Biology, Pathogenesis, and Immunity, Ed. R. S. Stephens, Washington (1999), P. 139–169.
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Translated from Byulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 146, No. 8, pp. 204–209, August, 2008
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Shkarupeta, M.M., Kostrjukova, E.S., Lazarev, V.N. et al. Localization of C. trachomatis inc proteins in expression of their genes in HeLa cell culture. Bull Exp Biol Med 146, 237–242 (2008). https://doi.org/10.1007/s10517-008-0254-8
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DOI: https://doi.org/10.1007/s10517-008-0254-8