The impact of culturing conditions (multiplicity of cell culture infection with influenza virus, composition of growth and maintenance nutrient media) for the efficiency of multiplication of cold-adapted reassortant vaccine strains of influenza A and B viruses was evaluated. Soybean hydrolysate protein-based biological additive to nutrient medium provided effective reproduction of influenza A virus in MDCK cells in the presence of 2 µg/ml trypsin. The use of soybean peptone-based stabilizer provided retention of infectious titer of influenza virus grown in MDCK culture after its lyophilization to a level of 8.5 lg EID50/ml.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Instruction for Evaluation of Shelf Life of Drugs by the “Rapid Aging” Method at High Temperature [in Russian], Ministry of Health of the USSR, Moscow (1983).
N. A. Mazurkova, T. Yu. Sen'kina, T. M. Sviridenko, et al., Biotekhnologiya, No. 3, 58–64 (2003).
R. Brands, J. Visser, J. Medema, et al., Dev. Biol. Stand., 98, 93–100 (1999).
P. Bruhl, A. Kerschbaum, O. Kistner, et al., Vaccine, 19, Nos. 9–10, 1149–1158 (2000).
P. Castle and J. S. Robertson, Dev. Biol. Stand., 99, 191–196 (1999).
Y. Z. Gendon, S. G. Markushin, I. I. Akopova, et al., Vaccine, 23, No. 38, 4678–4684 (2005).
N. Kessler, G. Thomas-Roche, L. Gerentes, M. Aymard, Dev. Biol. Stand., 98, 13–21 (1999).
O. Kistner, P. Barrett, W. Mundt, et al., Vaccine, 16, Nos. 9–10, 960–968 (1998).
O. W. Merten, J. C. Manuguerra, C. Hannoun, and S. Van der Werf, Dev. Biol. Stand., 98, 23–37 (1999).
A. M. Palache, H. S. Scheepers, V. de Regt, et al., Ibid., 98, 115–125 (1999).
J. Robertson, Virology, 3, 97–106 (1993).
E. Roca, X. Xo, and H. Haw, J. Gen. Virol., 74, 2513–2518 (1993).
W. W. Thompson, D. K. Shay, and E. Weintraub, JAMA, 289, No. 2, 179–186 (2003).
P. F. Wright and R. Webster, Orthomyxoviruses in Field Virology, Eds. D. Knipe and P. Howley, Philadelphia (2001).
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Kletochnye Tehnologii v Biologii i Medicine, No. 3, pp. 157–160, August, 2008
Rights and permissions
About this article
Cite this article
Mazurkova, N.A., Kolokol’tsova, T.D., Nechaeva, E.A. et al. The use of components of plant origin in the development of production technology for live cold-adapted cultural influenza vaccine. Bull Exp Biol Med 146, 144–147 (2008). https://doi.org/10.1007/s10517-008-0223-2
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-008-0223-2