We studied the presence of colony-forming cells in cell culture from rat heart 40 days after experimental myocardial infraction. The mean cellularity in this pathology was 12±8 cell/cm2, which is 20-fold lower than in intact myocardium. Transplantation of mesenchymal stem cells into the remodeling myocardium restored the pool of colony-forming cells. This effect depended on the state of transplanted cells. After transplantation of mesenchymal stem cells with low content of stress proteins, 6±2 colonies were detected, while after transplantation of cells with high content of hsp70 and hsp60 stress proteins (modified mesenchymal stem cells) 18±5 colonies were found, the mean cellularity of the corresponding cultured being 946±267 and 1926±123 cell/cm2. The positive effect of modified mesenchymal stem cells was observed on days 4 and 7 after transplantation. We conclude that postinfarction remodeling mobilized the total pool of regional stem cells; mesenchymal stem cells with high content of hsp70 and hsp60 demonstrated highest survival rate after intramyocardial transplantation.
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Translated from Kletochnye Tehnologii v Biologii i Medicine, No. 3, pp. 123–127, August, 2008
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Afanasiev, S.A., Falaleeva, L.P., Rebrova, T.U. et al. Effect of stress-proteins on survival of bone marrow mesenchymal stem cells after intramyocardial transplantation against the background of postinfarction heart remodeling. Bull Exp Biol Med 146, 111–115 (2008). https://doi.org/10.1007/s10517-008-0215-2
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DOI: https://doi.org/10.1007/s10517-008-0215-2