We studied the effect of succinic acid on the process of glucuronidation of 2-ethyl-6-methyl-3-hydroxypyridine after peroral and intraperitoneal administration in the form of succinate or a base. Since the basic form of 2-ethyl-6-methyl-3-hydroxypyridine is insoluble in water, it was administered in 5% Tween-80. It was necessary to evaluate also the effect of Tween-80 on glucuronidation of 2-ethyl-6-methyl-3-hydroxypyridine in different administration routes. Quantitative assay of glucuronidated fractions was performed by the method of reversed-phase HPLC with fluorometrical detection. The detection limit for this method was 10 ng/ml. We confirmed that the major excretion pathway for 2-ethyl-6-methyl-3-hydroxypyridine is conjugation with glucuronic acid. It was found that succinic acid increased excretion of glucuronidated metabolite after both peroral and intraperitoneal administration of 2-ethyl-6-methyl-3-hydroxypyridine in the form of succinate and base in 5% Tween-80. The effect of Tween-80 was detected only after peroral administration, which was probably related to its effect on absorption of this compound. Tween-80 increased excretion of glucuronate after peroral administration of 2-ethyl-6-methyl-3-hydroxypyridine in the form of succinate and in 5% Tween solution.
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I. I. Miroshnichenko, S. Yu. Kuz'mina, A. E. Voronina, Byull. Vseross. Nauch. Tcentra Biologicheski-Activnykh Veshchestv, No. 2, 49–54 (1994).
A. K. Sariev, V. P. Zherdev, A. A. Litvin, et al., Eksp. Klin. Farmakol., 62, No. 5, 42–46 (1999).
V. G. Kukes, Metabolism of Drugs: Clinical and Pharmacological Aspects [in Russian], Moscow (2004).
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Translated from Byulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 146, No. 7, pp. 62–64, July, 2008
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Baranov, P.A., Kravtsova, O.U., Sariev, A.K. et al. Effect of succinic acid and Tween-80 on glucuronidation of 2-ethyl-6-methyl-3-hydroxypyridine. Bull Exp Biol Med 146, 56–58 (2008). https://doi.org/10.1007/s10517-008-0205-4
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DOI: https://doi.org/10.1007/s10517-008-0205-4