Abstract
Peptide agonist of PAR1 in a concentration of 10 µM significantly facilitated neuromuscular transmission in newly formed synapses in mice. The absence of changes in the amplitude of miniature end-plate potentials attests to presynaptic mechanism of the effect of PAR1 agonist. The effect of the peptide was blocked by protein kinase A inhibitor H89 (1 µM). Blockade of inositol-1,4,5-trisphosphate receptors with 2-aminoethoxydiphenylborate (30 µM) did not prevent the effects of PAR1 agonist. Inhibition of protein kinase C with bisindolylmaleimide (1 µM) facilitated neuromuscular transmission in newly formed synapses. Protein kinase C inhibition was associated with reversal of the object of PAR1 agonist: transmission inhibition instead of facilitation.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 144, No. 11, pp. 490–494, November, 2007
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Balezina, O.P., Gerasimenko, N.Y. & Strukova, S.M. Effect of PAR1 agonist on acetylcholine secretion in a newly formed neuromuscular synapse in mice. Bull Exp Biol Med 144, 653–656 (2007). https://doi.org/10.1007/s10517-007-0396-0
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DOI: https://doi.org/10.1007/s10517-007-0396-0