Abstract
We studied the effects of antibacterial peptides and proteins (defensins and lactoferrins) on functional activity of endothelial cells in vitro: proliferative activity and adhesion of human endothelial ECV-304 cells to the matrix were evaluated. α-Defensin (NP-2) from rabbit neutrophils, total α-defensin (HNP 1-3) from human neutrophils, and lactoferrins from porcine neutrophils and human milk were studied. Defensins stimulated and lactoferrin in doses of 1–10 µg/ml inhibited proliferation and adhesion of endothelial cell. The stimulatory effect of defensins on proliferation and adhesion was reproduced in fibroblast culture. Lactoferrins did not modify proliferation of fibroblasts, but suppressed their adhesion. These data suggest that antibiotic proteins and peptides are prospective objects for the creation of drugs regulating angiogenesis.
Similar content being viewed by others
References
T. S. Bryazzhikova, G. M. Alyoshina, V. A. Isakov, et al., Byull. Eksp. Biol. Med., 120, No. 9, 329–331 (1995).
V. N. Kokryakov, Essays on Congenital Immunity [in Russian], St. Petersburg (2006).
B. A. Kudryashov, M. V. Kondashevskaya, L. A. Lyapina, et al., Byull. Eksp. Biol. Med., 109, No. 4, 391–393 (1990).
Yu. V. Chaly, E. M. Paleolog, T. S. Kolesnikova, et al., Eur. Cytokine Netw., 11, No. 2, 257–266 (2000).
T. Chavakis, D. B. Cines, J. S. Rhee, et al., FASEB J., 18, No. 11, 1306–1308 (2004).
A. A. Higazi, T. Ganz, K. Kariko, and D. B. Cines, J. Biol. Chem., 271, No. 30, 17650–17655 (1966).
C. W. Kim, K. N. Son, S. Y. Choi, and J. Kim, FEBS Lett., 580, No. 18, 4332–4336 (2006).
K. Norrby, I. Mattsby-Baltzer, M. Innocenti, and S. Tuneberg, Int. J. Cancer, 91, No. 2, 236–240 (2001).
A. V. Panyutich, E. A. Panyutich, V. A. Krapivin, et al., J. Lab. Clin. Med., 122, No. 2, 202–207 (1993).
K. Sakamoto, Y. Ito, T. Mori, and K. Sugimura, J. Biol. Chem., 281, No. 34, 24272–24278 (2006).
Y. A. Suzuki, V. Lopez, and B. Lunnerdal, Cell. Mol. Life Sci., 62, No. 22, 2560–2575 (2005).
Author information
Authors and Affiliations
Corresponding author
Additional information
__________
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 144, No. 9, pp. 306–309, September, 2007
Rights and permissions
About this article
Cite this article
Krylov, A.V., Kisseleva, E.P., Aleshina, G.M. et al. Effects of defensin and lactoferrin on functional activity of endothelial cells in vitro . Bull Exp Biol Med 144, 331–334 (2007). https://doi.org/10.1007/s10517-007-0325-2
Received:
Issue Date:
DOI: https://doi.org/10.1007/s10517-007-0325-2