Abstract
Enzyme immunoassay showed that blood serum from healthy donors contains specific high-affinity antibodies (apparent association constant ≥5×109 M–1) against a fragment of transcribed region of ribosomal DNA repeat of human serum, which are present in a free form or are bound to extracellular DNA. Preheating of the serum at 55°C and high ionic strength (1.5 M NaCl) had no effect on the interaction of antibodies with this fragment. Competitive binding assay showed that these antibodies recognize DNA epitopes, which differ from the epitopes recognized by most anti-DNA antibodies in systemic lupus erythematosus.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 144, No. 9, pp. 277–281, September, 2007
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Veiko, N.N., Kostyuk, S.V., Ermakov, A.V. et al. Peripheral blood serum from healthy donors contains antibodies against the fragment of transcribed region of ribosomal repeat. Bull Exp Biol Med 144, 304–308 (2007). https://doi.org/10.1007/s10517-007-0317-2
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DOI: https://doi.org/10.1007/s10517-007-0317-2