Abstract
We developed a technology of labeling bone marrow precursor cells with the Lin—c-kit+ phenotype in culture by green fluorescent protein gene using a lentivirus vector. The proposed system provides effective transduction of bone marrow precursor cells and high transgene expression level in vitro (27%). The integration of the transgene into the transduced cell genome in vivo was verified by the method of splenic colonies.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 143, No. 6, pp. 667–671, June, 2007
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Radyukhina, N.V., Rutkevich, P.N., Aref’yeva, T.I. et al. Lentivirus transduction of bone marrow hemopoietic precursor cells with Lin-c-kit+ phenotype Ex Vivo using a genetic construct containing green fluorescent protein gene. Bull Exp Biol Med 143, 723–726 (2007). https://doi.org/10.1007/s10517-007-0224-6
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DOI: https://doi.org/10.1007/s10517-007-0224-6