Abstract
A cDNA fragment encoding GFAP was amplified by reverse transcription PCR from total mRNA isolated from primary culture of rat astrocytes and cloned for expression in Escherichia coli using pET-28a vector. High level of GFAP expression was confirmed by SDS-PAGE, while immunochemical identity was verified by immunoblotting. The constructed producer strain is a cheap source of GFAP and can be used for diagnostic purposes.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 143, No. 1, pp. 74–77, January, 2007
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Chekhonin, V.P., Pavlov, K.A., Shkoporov, A.N. et al. Cloning and expression of rat GFAP cDNA in Escherichia coli . Bull Exp Biol Med 143, 68–71 (2007). https://doi.org/10.1007/s10517-007-0019-9
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DOI: https://doi.org/10.1007/s10517-007-0019-9