Abstract
Specimens of human serum γ-globulin modified by molar excess of copper and zinc cations were obtained by molecular ultrafiltration. Conformation characteristics of the protein were determined by UV spectrophotometry. Immunochemical study included radial immunodiffusion test and direct and sandwich enzyme-linked immunosorbent assay. After binding of copper and zinc, the γ-globulin molecule underwent conformation changes modifying presentation of antigenic determinants on the globule surface and their availability for recognition by specific antibodies. The effects of copper were much more pronounced than those of zinc cations.
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References
S. L. Grokhovskii, V. A. Nikolaev, V. E. Zubarev, et al., Mol. Biol., 26, No. 6, 1274–1297 (1992).
A. Ya. Kul’berg, Antiimmunoglobulins [in Russian], Moscow (1978).
K. A. Markosyan and B. I. Kurganov, Biokhimiya, 68, No. 8, 1013–1025 (2003).
V. N. Rakitskii and T. V. Yudina, Vestn. Rossiisk. Akad. Med. Nauk, No. 3, 33–36 (2005).
S. V. Tkachyov and A. A. Ushkov, Byull. Eksp. Biol. Med., 140, No. 9, 291–294 (2005).
S. B. Cheknyov, E. E. Babaeva, U. A. Vorobyova, and E. A. Denisova, Med. Immunologiya, 7, No. 4, 375–380 (2005).
S. B. Cheknyov, E. E. Babaeva, M. S. Zharkova, et al., Byull. Eksp. Biol. Med., 139, No. 2, 185–187 (2005).
V. Chelia and T. Blandell, Biokhimiya, 70, No. 8, 1013–1020 (2005).
R. Bauer, A. Muller, M. Richter, et al., Biochim. Biophys. Acta, 1334, No. 1, 98–108 (1997).
J. M. Berg, J. Biol. Chem., 265, No. 12, 6513–6516 (1990).
D. B. Borza and W. T. Morgan, Ibid., 273, No. 10, 5493–5499 (1998).
A. L. Jones, M. D. Hulett, and C. R. Parish, Ibid., 279, No. 29, 30,114–20,122 (2004).
N. S. Greenspan and L. J. N. Cooper, Immunol. Today, 13, No. 5, 164–168 (1992).
M. A. Smith, M. Easton, P. Everett, et al., Int. J. Pept. Protein Res., 48, No. 1, 48–55 (1996).
W. W. Stewart, R. L. Mazengera, Shen Li, and M. A. Kerr, J. Leukoc. Biol., 56, No. 4, 481–487 (1994).
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 142, No. 12, pp. 666–670, December, 2006
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Cheknev, S.B., Babaeva, E.E., Denisova, E.A. et al. Preparation of specimens of human serum γ-globulin modified by copper and zinc and its immunochemical characteristics. Bull Exp Biol Med 142, 710–713 (2006). https://doi.org/10.1007/s10517-006-0458-8
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DOI: https://doi.org/10.1007/s10517-006-0458-8