Abstract
Long-term treatment of transformed 3T3-SV40 mouse fibroblasts with antioxidant N-acetylcysteine decreased cell level of ROS and increased the concentration of reduced glutathione. Removal of N-acetylcysteine from the medium led to the appearance of well-expressed stress fibrils, virtually absent in control cells. In contrast to control cells, these cells were not invaded by apathogenic Escherichia coli A2 strain producing ECP32 protease specifically cleaving actin. Antioxidant N-acetylcysteine can cause partial reversion of transformed phenotype at the expense of a shift of cell redox balance in favor of reduced glutathione.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
I. A. Gamalei, N. D. Aksyonov, T. N. Efremova, and K. M. Kirpichnikova, Tsitologiya, 45, No. 1, 26–33 (2003).
T. N. Efremova, K. M. Kirpichnikova, S. Yu. Khaitlina, and I. A. Gamalei, Ibid., 46, No. 5, 395–403 (2004).
P. Cossart and P. J. Sansonetti, Science, 304, No. 5668, 242–248 (2004).
P. N. Dekhuijzen, Eur. Respir. J., 23, No. 4, 629–636 (2004).
T. N. Efremova, N. Ender, M. Brudnaja, et al., Cell. Biol. Intern., 25, No. 4, 557–561 (2001).
X. A. Gibson, A. Shartava, J. McIntyre, et al., Blood, 91, No. 11, 4373–4378 (1998).
M. Kirk and S. Pace, Emerg. Med. Clin. North. Am., 15, No. 2, 427–449 (1997).
L. Kum-Tatt and I. K. Tan, Clin. Chim. Acta, 53, No. 2, 153–161 (1974).
J. F. Oram, A. J. Mendez, J. Lymp, et al., J. Lipid Res., 40, No. 10, 1769–1781 (1999).
M. Tyunder, L. Susini, S. Prieur, et al., Proc. Natl. Acad. Sci. USA, 99, No. 23, 14,976–14,981 (2002).
L. Tylicki, B. Tutkowski, and W. H. Horl, Kidney Blood Press. Res., 26, Nos. 5–6, 303–314 (2003).
P. Velge, E. Bottreau, B. Kaeffer, and P. Pardon, Med. Microbiol. Immunol., 183, No. 3, 145–158 (1994).
P. Velge, E. Bottreau, N. Van-Langendonck, and B. Kaeffer, J. Med. Microbiol., 46, No. 8, 681–692 (1997).
I. T. Virlos, J. Mason, D. Schoefield, et al., Scand. J. Gastroenterol., 38, No. 12, 1262–1267 (2003).
M. Zafarullah, W. Q. Li, J. Sylvester, and M. Ahmad, Cell. Mol. Life Sci., 60, No. 1, 6–20 (2003).
Author information
Authors and Affiliations
Corresponding author
Additional information
__________
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 142, No. 7, pp. 101–105, July, 2006
Rights and permissions
About this article
Cite this article
Gamalei, I.A., Efremova, T.N., Kirpichnikova, K.M. et al. Decreased sensitivity of transformed 3T3-SV40 cells treated with N-acetylcysteine to bacterial invasion. Bull Exp Biol Med 142, 90–93 (2006). https://doi.org/10.1007/s10517-006-0300-3
Received:
Issue Date:
DOI: https://doi.org/10.1007/s10517-006-0300-3