Skip to main content
  • Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
  • Published:

Study of growth parameters and potentialities of differentiation of multipotent mesenchymal stromal cells from rat bone marrow in vitro

Bulletin of Experimental Biology and Medicine volume 141, pages 530–535 (2006)Cite this article

Abstract

The dynamics of growth and proliferative activity of the population of multipotent mesenchymal stromal cells from rat bone marrow was studied during 7 passages. The efficiency of colony formation, the morphology of multipotent mesenchymal stromal cells, and the possibility of spontaneous and induced differentiation were studied. The rat bone marrow fibroblast-like multipotent mesenchymal stromal cells are capable of clonal growth; their proliferative activity and the yield remained high until passage 4, but then decreased. Induction of osteo-or adipogenic differentiation of bone marrow multipotent mesenchymal stromal cells increased the percentage of morphologically modified cells carrying specific markers.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

43,34 €

Price includes VAT (Finland)
Tax calculation will be finalised during checkout.

References

  1. E. Pearce, Histochemistry [in Russian], Moscow (1962), pp. 275, 365–367.

  2. T. Kh. Fatkhutdinov, D. V. Gol’dstein, A. A. Kulakov, et al., Kletochn. Tekhnol. Biol. Med., 2, 75–83 (2005).

    Google Scholar 

  3. P. Bianco, M. Riminucci, S. Gronthos, et al., Stem Cells, 19, 180–192 (2001).

    PubMed  CAS  Article  Google Scholar 

  4. J. S. Boo, Y. Yamada, J. Okazaki, et al., J. Craniofac. Surg., 13, No. 2, 231–239 (2002).

    PubMed  Article  Google Scholar 

  5. J. E. Grove, E. Bruscia, and D. S. Krause, Stem Cells, 22, 487–500 (2004).

    PubMed  Article  Google Scholar 

  6. E. H. Javazon, D. C. Colter, E. J. Schwarz, and D. J. Prockop, Ibid., 19, 219–225 (2001).

    PubMed  CAS  Article  Google Scholar 

  7. M. Kucia, R. Reca, V. R. Jala, et al., Leukemia, 19, 1118–1127 (2005).

    PubMed  CAS  Article  Google Scholar 

  8. E. Martin-Rendon and S.M. Watt, Brit. J. Haematol., 122, 877–891 (2003).

    Article  Google Scholar 

  9. L. S. Meirelles and N. B. Nardi, Ibid., 123, 702–701.

  10. Y. A. Romanov, V. A. Svintsitskaya, and V. N. Smirnov, Stem Cells, 21, 190–199 (2003).

    Article  Google Scholar 

  11. V. Vacanti, E. Kong, G. Suzuki, et al., J. Cell. Physiol., 205, No. 2, 194–201 (2005).

    PubMed  CAS  Article  Google Scholar 

  12. M. Yoshida, Eur. Surg. Res., 35, 86–91 (2003).

    PubMed  CAS  Article  Google Scholar 

  13. P. A. Zuk, M. Lhu, H. Mizuno, et al., Tissue Eng., 7, 211–228 (2001).

    PubMed  CAS  Article  Google Scholar 

Download references

Author information

Affiliations

  1. Department of Prediction of Conservative Treatment Efficiency, P. A. Hertzen Oncological Institute, Russian Ministry of Health, Russia

    N. S. Sergeeva, I. K. Sviridova, V. A. Kirsanova, S. A. Akhmedova & N. V. Marshutina

  2. N. I. Pirogov Russian State Medical University, Russia

    V. S. Sergeeva

  3. Biological Faculty, M. V. Lomonosov Moscow State University, Russia

    D. Yu. Bednik

Authors
  1. N. S. Sergeeva
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. I. K. Sviridova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. V. A. Kirsanova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S. A. Akhmedova
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. N. V. Marshutina
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. V. S. Sergeeva
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. D. Yu. Bednik
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to N. S. Sergeeva.

Additional information

__________

Translated from Kletochnye Tekhnologii v Biologii i Medicine, No. 2, pp. 102–107, April, 2006

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Sergeeva, N.S., Sviridova, I.K., Kirsanova, V.A. et al. Study of growth parameters and potentialities of differentiation of multipotent mesenchymal stromal cells from rat bone marrow in vitro . Bull Exp Biol Med 141, 530–535 (2006). https://doi.org/10.1007/s10517-006-0214-0

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10517-006-0214-0

Key Words

  • multipotent mesenchymal stromal cells
  • passage characteristics
  • differentiation