Abstract
Comparative analysis of differentiation of human neural and mesenchymal stem cells in tissue culture and after transplantation into the brain was carried out using the same antibody set. Neural stem cells differentiated into all types of neural cells, are retained after transplantation, migrate, and form reciprocal relationships with the recipient brain. Mesenchymal stem cells were incapable of neural development under conditions of common culturing or after transplantation and retained the fibroblast-like status. Recipient filaments grew into mesenchymal stem cell transplants containing no neural cells due to local changes in the extracellular matrix at the site of transplantation.
Similar content being viewed by others
References
S. A. Azizi, D. Stokes, B. J. Augelli. et al., Proc. Natl. Acad. Sci. USA., 95, 3908–3913 (1998).
B. J. Cummings, N. Uchida, S. J. Tamaki, et al., Ibid., 102, No. 39, 14,069–14,074 (2005).
M. Dezawa, H. Kano, M. Hoshino, et al., J. Clin. Invest., 113, No. 12, 1701–1710 (2004).
K. Jin, M. Xo, S. Batteur, et al., Ibid., 184, No. 1, 78–89 (2003).
K. Kurozumi, K. Nakamura, T. Tamiya, et al., Mol. Ther., 11, No. 1, 96–104 (2005).
X. Long, M. Olszewski, W. Huang, et al., Stem Cells Dev., 14, No. 1, 65–69 (2005).
P. Lu, A. Blesch, and M. H. Tuszynski, J. Neurosci. Res., 77, No. 2, 174–191 (2004).
P. Lu, L. L. Jones, and M. H. Tuszynski, Exp. Neurol., 191, No. 2, 334–360.
T. Magaki, K. Kurisu, T. Okazaki, Neurosci. Lett., 384, No. 3, 282–287 (2005).
A. Mahmood, D. Lu, and M. Chopp J., Neurotrauma, 21, No. 1, 33–39 (2004).
J. J. Minguell, F. A. Fierro, M. J. Epunan, et al., Stem Cells Dev., 14, No. 4, 408–414 (2005).
K. Mori, J. Iwata, M. Miyazaki, et al., J. Cereb. Blood Flow Metab., 25, No. 7, 887–898 (2005).
G. Munoz-Elias, A. J. Marcus, T. M. Coyne, et al., J. Neurosci., 24, No. 19, 4585–4595 (2004).
B. Neuhuber, G. Gallo, L. Howard, et al., J. Neurosci. Res., 77, No. 2, 192–204 (2004).
B. Neuhuber, B. Timothy Himes, J. S. Shumsky et al., Brain Res., 1035, No. 1, 73–85 (2005).
A. L. Pearlman and A. M. Sheppard, Prog. Brain Res., 108, 117–134 (1996).
C. M. Sahlgren, A. Mikhailov, S. Vaittinen, et al., Mol. Cell Biol., 23, No 14, 5090–5106 (2003).
H. Tao, R. Rao, and D.D. Ma, Dev. Growth. Differ., 47, No. 6, 423–433 (2005).
T. Tondreau, L. Lagneaux, M. Dejeneffe, et al., Differentiation, 72, No. 7, 319–326 (2004).
J. Wang and R. Milner, J. Neurochem., 96, No. 1, 148–159 (2006).
T. Wehner, M. Bontert, I. Eyupoglu, et al., J. Neurosci., 23, No. 12, 5004–5011 (2003).
S. Wislet-Gendebien, F. Bruyere, G. Hans, et al., BMC Neurosci., 5, No. 1, 1–33 (2004).
D. Woodbury, E. J. Schwarz, D. J. Prockop, et al., J. Neurosci. Res., 61, No. 4, 364–370 (2000).
J. Zhang, Y. Li, J. Chen, et al., Brain Res., 1030, No. 1, 19–27 (2004).
L. R. Zhao, W. M. Duan, M. Reyes, et al., Exp. Neurol., 174, No. 1, 11–20 (2002).
Author information
Authors and Affiliations
Corresponding author
Additional information
__________
Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 1, pp. 44–52, January, 2006
Rights and permissions
About this article
Cite this article
Aleksandrova, M.A., Sukhikh, G.T., Chailakhyan, R.K. et al. Comparative analysis of differentiation and behavior of human neural and mesenchymal stem cells In Vitro and In Vivo . Bull Exp Biol Med 141, 152–160 (2006). https://doi.org/10.1007/s10517-006-0116-1
Received:
Issue Date:
DOI: https://doi.org/10.1007/s10517-006-0116-1