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Differential Diagnosis of Various Forms of Myasthenia and Endocrine Ophthalmopathy by Immunoblotting

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Abstract

Immunoblotting can be used for screening a population of antibodies to acetylcholine receptor subunits circulating in the blot of patients with myasthenia. Torpedo Californica acetylcholine receptor served as the antigen. We found that in generalized myasthenia autoantibodies bind to α1- or α1- and γ-subunits, while in ophthalmic form they bind only γ-subunit of acetylcholine receptor. No antibodies to any of the acetylcholine receptor subunits were detected in patients with endocrine ophthalmopathy and in healthy volunteers. This method can be used for differential diagnosis of ophthalmic myasthenia and endocrine ophthalmopathy and for predicting generalization of the pathological process in patients with myasthenia.

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Authors and Affiliations

  1. Department of Human Neuromuscular Diseases, Institute of Pathology and Pathophysiology, Russian Academy of Medical Sciences, Moscow, Russia

    V. B. Lantsova & E. K. Sepp

Authors
  1. V. B. Lantsova
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  2. E. K. Sepp
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Translated from Byulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 140, No. 10, pp. 478–480, October, 2005

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Lantsova, V.B., Sepp, E.K. Differential Diagnosis of Various Forms of Myasthenia and Endocrine Ophthalmopathy by Immunoblotting. Bull Exp Biol Med 140, 473–475 (2005). https://doi.org/10.1007/s10517-005-0522-9

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  • DOI: https://doi.org/10.1007/s10517-005-0522-9

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