Skip to main content
Log in

Mesenchymal Stem Cells from Human Bone Marrow and Adipose Tissue: Isolation, Characterization, and Differentiation Potentialities

  • Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

Abstract

Comparative study of cultured human bone marrow and adipose tissue (lipoaspirate) mesenchymal stem cells was carried out. The main morphological parameters, proliferative activity, expression of surface and intracellular markers of these cells were characterized. Flow cytofluorometry and histological staining showed that both cell types exhibited similar expression of CD105, CD54, CD106, HLA-I markers, were positively stained for vimentin, ASMA, collagen-1, and fibronectin, but not HLA-DR, CD117, and hemopoietic cell markers. The cells underwent differentiation into adipocytes and osteoblasts under appropriate conditions of culturing. Incubation under neuroinductive conditions led to the appearance of a cell population positively stained for type III β-tubulin (neuronal differentiation marker).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

REFERENCES

  1. A. Alhadlaq and J. J. Mao, Stem Cell Dev., 13, 436–448 (2004).

    Article  Google Scholar 

  2. L. Aust, B. Devlin, S. Foster, et al., Cytotherapy, 6, 7–14 (2004).

    Article  PubMed  Google Scholar 

  3. P. Bianco and P. G. Robey, J. Clin. Invest., 105, 1663–1668 (2000).

    PubMed  Google Scholar 

  4. D. Clarke and J. Frisen, Curr. Opin. Gen. Dev., 11, 575–580 (2001).

    Article  Google Scholar 

  5. D. Covas, J. Siufi, A. Silva, and M. Orellana, Braz. J. Med. Biol. Res., 36, 1179–1183 (2003).

    Article  PubMed  Google Scholar 

  6. S. J. Forbes, P. Vig, R. Poulsom, et al., Clin. Sci., 103, 355–369 (2002).

    PubMed  Google Scholar 

  7. A. J. Fridenstein, U. F. Deriglazova, N. N. Kulagina, et al., Exp. Hematol., 2, 83–92 (1974).

    PubMed  Google Scholar 

  8. Y. Fukuchi, H. Nakajima, D. Sugiyama, et al., Stem Cells, 22, 649–658 (2004).

    Article  PubMed  Google Scholar 

  9. J. E. Grove, E. Bruscia, and D. S. Krause, Ibid., 487–500.

  10. S. Hung, C. Cheng, C. Pan, et al., Ibid., 20, 522–529 (2002).

    Article  PubMed  Google Scholar 

  11. A. J. Katz, A. Tholpady, S. Tholpady, et al., Ibid., 23, 412–423 (2005).

    Article  PubMed  Google Scholar 

  12. D. S. Krause, Gene Ther., 9, 754–758 (2002).

    Article  PubMed  Google Scholar 

  13. K. E. Mitchell, M. L. Weiss, B. M. Mitchell, et al., Stem Cells, 21, 50–60 (2003).

    PubMed  Google Scholar 

  14. Y. A. Romanov, V. B. Svintsitskaya, and V. N. Smirnov, Ibid., 105–110.

  15. R. S. Tuan, G. Boland, and R. Tuli, Arthritis Res. Ther., 5, 32–45 (2002).

    Article  PubMed  Google Scholar 

  16. H. Wang, S. Hung, S. Peng, et al., Stem Cells, 22, 1330–1337 (2004).

    Article  PubMed  Google Scholar 

  17. G. G. Wulf, K. A. Jackson, and M. A. Goodell, Exp. Hematol., 29, 1361–1370 (2001).

    Article  PubMed  Google Scholar 

  18. P. A. Zuk, M. Zhu, P. Ashjian, et al., Mol. Biol. Cell, 13, 4279–4295 (2002).

    Article  PubMed  Google Scholar 

  19. P. A. Zuk, M. Zhu, H. Mizuno, et al., Tissue Eng., 7, 211–228 (2001).

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Yu. A. Romanov.

Additional information

__________

Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 3, pp. 158–163, September, 2005

Rights and permissions

Reprints and permissions

About this article

Cite this article

Romanov, Y.A., Darevskaya, A.N., Merzlikina, N.V. et al. Mesenchymal Stem Cells from Human Bone Marrow and Adipose Tissue: Isolation, Characterization, and Differentiation Potentialities. Bull Exp Biol Med 140, 138–143 (2005). https://doi.org/10.1007/s10517-005-0430-z

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10517-005-0430-z

Key Words

Navigation