Abstract
Cultured mouse embryonic stem cells can be transfected with a reporter gene encoding blue fluorescent protein BFP and regulated by drosophila heat shock protein 70 promoter. This gene is activated after heating and synthesizes matrix RNA. Blue protein is synthesized under these conditions. The system for transfection of stem cells allows us to activate automatically the corresponding transgenes.
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Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 2, pp. 99–102, 2005
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Pavlova, G.V., Manuilova, E.S., Arsen’eva, E.L. et al. BFP Protein Expression in Transfected Embryonic Stem Cells. Bull Exp Biol Med 139, 514–516 (2005). https://doi.org/10.1007/s10517-005-0333-z
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DOI: https://doi.org/10.1007/s10517-005-0333-z