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Phagocytic activity of neutrophils and lymphocytes at various periods of storage in the state of cold anabiosis (-40°C)

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Abstract

We studied changes in phagocytic activity of neutrophils and lymphocytes subjected to cold anabiosis. The proposed effective method for introduction of human blood leukocytes into cold anabiosis at moderately low temperature (-40°C) in the presence of a cryopreserving agent does not require washout of the biological material after defrosting.

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REFERENCES

  1. A. M. Belous and V. I. Grishchenko, Cryobiology [in Russian], Kiev (1994).

  2. S. Glants, Medical and Biological Statistics [in Russian], Moscow (1998).

  3. A. M. Goldovskii, Anabiosis and Its Practical Importance [in Russian], Leningrad (1986).

  4. S. G. Potapova, V. S. Khrustikov, N. V. Demidova, and G. I. Kozinets, Probl. Gematol. Perelivanija Krovi, No. 9, 58–59 (1977).

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Authors and Affiliations

  1. Laboratory for Cryophysiology of Blood, Institute of Physiology, Komi Research Center, Ural Division of the Russian Academy of Sciences, Syktyvkar

    E. P. Svedentsov, O. O. Shcheglova, T. V. Tumanova, O. N. Devet’yarova, A. A. Kostyaev & S. V. Utemov

  2. Laboratory for Conservation of Blood and Tissues, Kirov Institute of Hematology and Blood Transfusion, Russian Ministry of Health, Russia

    A. A. Kostyaev & S. V. Utemov

Authors
  1. E. P. Svedentsov
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  2. O. O. Shcheglova
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  3. T. V. Tumanova
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  4. O. N. Devet’yarova
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  5. A. A. Kostyaev
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  6. S. V. Utemov
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Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 138, No. 10, pp. 400–402, October, 2004

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Svedentsov, E.P., Shcheglova, O.O., Tumanova, T.V. et al. Phagocytic activity of neutrophils and lymphocytes at various periods of storage in the state of cold anabiosis (-40°C). Bull Exp Biol Med 138, 354–356 (2004). https://doi.org/10.1007/s10517-005-0098-4

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  • DOI: https://doi.org/10.1007/s10517-005-0098-4

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