Abstract
Culturing of suspension fraction of a long-term bone marrow culture derived from tumor necrosis factor (TNF)-deficient mice for 75 days produced cells forming adherent cell strains. The cells of all strains expressed RNA of various stromal differentiating markers in various combinations. The cells of many strains simultaneously expressed genes encoding products characteristic of different differentiation lineages. The derived strains maintained hemopoiesis for 10 weeks. RNA-analysis of gene expression by cells of these strains showed that they express a set of various growth factors and cytokines. It was hypothesized that suspension fraction of long-term bone marrow culture derived from TNF-deficient mice includes immature stromal precursor cells, which were never detected in long-term bone marrow culture derived from wild-type mice.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 138, No. 9, pp. 284–288, September, 2004
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Nifontova, I.N., Ol’shanskaya, Y.V. & Drize, N.I. Stromal cell strains derived from suspension fraction of bone marrow cultures of tumor necrosis factor-deficient mice. Bull Exp Biol Med 138, 251–254 (2004). https://doi.org/10.1007/s10517-005-0013-z
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DOI: https://doi.org/10.1007/s10517-005-0013-z