Abstract
In the past, diatoms were considered the primary food source for copepods in both wild and aquaculture settings. However, recent studies have found that diatoms have defense mechanisms against predators, making them unsuitable as copepod feed. This study assessed the impact of addition of 100 μg L−1 of silicate to an inorganic nutrition formula containing 700 μg L−1 nitrogen, 100 μg L−1 phosphorus, and 100 μg L−1 iron. Our objective was to assess the impact of increased diatom abundance on copepod production. The experiment was carried out in 1000 L outdoor tanks over a period of 20 days, with adult Pseudodiaptomus annandalei copepods, introduced into each tank on the second day at an initial density of 10 ind. L−1. The results indicated that adding silicate reduced the prevalence of Chlorophyta, replaced by a higher proportion of Dinophyta, and eventually dominated by diatoms. While silicate had a positive effect on diatom culture, it had a negative effect on copepod production. Adding silicate to the inorganic fertilization method resulted in increased costs, leading to a significant increase in the unit production cost of copepods.
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References
Aguirre LE, Ouyang L, Elfwing A, Hedblom M, Wulff A, Inganäs O (2018) Diatom frustules protect DNA from ultraviolet light. Sci Rep 8:1–6. https://doi.org/10.1038/s41598-018-21810-2
Andersen RA (2005) Algal culturing techniques. Elsevier, Cambridge
Asai S, Sanges R, Lauritano C, Lindeque PK, Esposito F, Ianora A, Carotenuto Y (2020) De novo transcriptome assembly and gene expression profiling of the copepod Calanus helgolandicus feeding on the PUA-producing diatom Skeletonema marinoi. Mar Drugs 18:392. https://doi.org/10.3390/md18080392
Begum M, Sahu BK, Das AK, Vinithkumar NV, Kirubagaran R (2015) Extensive Chaetoceros curvisetus bloom in relation to water quality in Port Blair Bay, Andaman Islands. Environ Monit Assess 187:1–14. https://doi.org/10.1007/s10661-015-4461-2
Berggreen U, Hansen B, Kiørboe T (1988) Food size spectra, ingestion and growth of the copepod Acartia tonsa during development: Implications for determination of copepod production. Mar Biol 99:341–352. https://doi.org/10.1007/BF02112126
Berk S, Brownlee D, Heinle D, Kling H, Colwell R (1977) Ciliates as a food source for marine planktonic copepods. Microb Ecol 4:27–40. https://doi.org/10.1007/BF02010427
Beyrend-Dur D, Kumar R, Rao TR, Souissi S, Cheng SH, Hwang JS (2011) Demographic parameters of adults of Pseudodiaptomus annandalei (Copepoda: Calanoida): temperature–salinity and generation effects. J Exp Mar Biol Ecol 404:1–14. https://doi.org/10.1016/j.jembe.2011.04.012
Blanda E, Drillet G, Huang CC, Hwang JS, Jakobsen HH, Rayner TA, Su HM, Wu CH, Hansen BW (2015) Trophic interactions and productivity of copepods as live feed from tropical Taiwanese outdoor aquaculture ponds. Aquaculture 445:11–21. https://doi.org/10.1016/j.aquaculture.2015.04.003
Blanda E, Drillet G, Huang CC, Hwang JS, Højgaard JK, Jakobsen HH, Rayner TA, Su HM, Hansen BW (2017) An analysis of how to improve production of copepods as live feed from tropical Taiwanese outdoor aquaculture ponds. Aquaculture 479:432–441. https://doi.org/10.1016/j.aquaculture.2017.06.018
Brugnano C, Granata A, Guglielmo L, Minutoli R, Zagami G, Ianora A (2016) The deleterious effect of diatoms on the biomass and growth of early stages of their copepod grazers. J Exp Mar Biol Ecol 476:41–49. https://doi.org/10.1016/j.jembe.2015.11.015
Calbet A, Saiz E (2005) The ciliate-copepod link in marine ecosystems. Aquat Microb Ecol 38:157–167. https://doi.org/10.3354/ame038157
Camus T, Zeng C, McKinnon AD (2009) Egg production, egg hatching success and population increase of the tropical paracalanid copepod, Bestiolina similis (Calanoida: Paracalanidae) fed different microalgal diets. Aquaculture 297:169–175. https://doi.org/10.1016/j.aquaculture.2009.09.018
Chen Q, Sheng J, Lin Q, Gao Y, Lv J (2006) Effect of salinity on reproduction and survival of the copepod Pseudodiaptomus annandalei Sewell, 1919. Aquaculture 258:575–582. https://doi.org/10.1016/j.aquaculture.2006.04.032
Das P, Mandal SC, Bhagabati SK, Akhtar MS, Singh SK (2012) Important live food organisms and their role in aquaculture. Front Aquac 5:69–86. https://doi.org/10.13140/RG.2.2.21105.07523
Delgado M, Alcaraz M (1999) Interactions between red tide microalgae and herbivorous zooplankton: the noxious effects of Gyrodinium corsicum (Dinophyceae) on Acartia grani (Copepoda: Calanoida). J Plankton Res 21:2361–2371. https://doi.org/10.1093/plankt/21.12.2361
Dortch Q, Whitledge TE (1992) Does nitrogen or silicon limit phytoplankton production in the Mississippi River plume and nearby regions? Cont Shelf Res 12:1293–1309. https://doi.org/10.1016/0278-4343(92)90065-R
Falkowski PG, Barber RT, Smetacek V (1998) Biogeochemical controls and feedbacks on ocean primary production. Science 281:200–206. https://doi.org/10.1126/science.281.5374.200
Golez MN, Takahashi T, Ishimarul T, Ohno A (2004) Post-embryonic development and reproduction of Pseudodiaptomus annandalei (Copepoda: Calanoida). Plankton Biology and Ecology 51:15–25
GrØnning J, Doan NX, Dinh NT, Dinh KV, Nielsen TG (2019) Ecology of Pseudodiaptomus annandalei in tropical aquaculture ponds with emphasis on the limitation of production. J Plankton Res 41:741–758. https://doi.org/10.1093/plankt/fbz053
Guillard RR, Ryther JH (1962) Studies of marine planktonic diatoms: I. Cyclotella nana Hustedt, and Detonula confervacea (Cleve) Gran. Can J Microbiol 8:229–239. https://doi.org/10.1139/m62-029
Hamm CE, Merkel R, Springer O, Jurkojc P, Maier C, Prechtel K, Smetacek V (2003) Architecture and material properties of diatom shells provide effective mechanical protection. Nature 421:841–843. https://doi.org/10.1038/nature01416
Han J, Park JS, Park Y, Lee J, Shin HH, Lee KW (2021) Effects of paralytic shellfish poisoning toxin-producing dinoflagellate Gymnodinium catenatum on the marine copepod Tigriopus japonicus. Mar Pollut Bull 163:111937. https://doi.org/10.1016/j.marpolbul.2020.111937
Hong GK, Tew KS (2022) The advantages of inorganic fertilization for the mass production of copepods as food for fish larvae in aquaculture. Life 12:441. https://doi.org/10.3390/life12030441
Hong GK, Kuo J, Tew KS (2023) Iron fertilization can enhance the mass production of copepod, Pseudodiaptomus annandalei, for fish aquaculture. Life 13:529. https://doi.org/10.3390/life13020529
Ianora A, Poulet SA, Miralto A (2003) The effects of diatoms on copepod reproduction: a review. Phycologia 42:351–363. https://doi.org/10.2216/i0031-8884-42-4-351.1
Ianora A, Miralto A, Poulet SA, Carotenuto Y, Buttino I, Romano G, Casotti R, Pohnert G, Wichard T, Colucci-D’Amato L (2004) Aldehyde suppression of copepod recruitment in blooms of a ubiquitous planktonic diatom. Nature 429:403–407. https://doi.org/10.1038/nature02526
Ianora A, Bastianini M, Carotenuto Y, Casotti R, Roncalli V, Miralto A, Romano G, Gerecht A, Fontana A, Turner JT (2015) Non-volatile oxylipins can render some diatom blooms more toxic for copepod reproduction. Harmful Algae 44:1–7. https://doi.org/10.1016/j.hal.2015.02.003
Irigoien X, Harris R, Head R, Harbour D (2000a) The influence of diatom abundance on the egg production rate of Calanus helgolandicus in the English Channel. Limnol Oceanogr 45:1433–1439. https://doi.org/10.4319/lo.2000.45.6.1433
Irigoien X, Head R, Harris R, Cummings D, Harbour D, Meyer-Harms B (2000b) Feeding selectivity and egg production of Calanus helgolandicus in the English Channel. Limnol Oceanogr 45:44–54. https://doi.org/10.4319/lo.2000.45.1.0044
Irigoien X, Harris RP, Verheye HM, Joly P, Runge J, Starr M, Pond D, Campbell R, Shreeve R, Ward P (2002) Copepod hatching success in marine ecosystems with high diatom concentrations. Nature 419:387–389. https://doi.org/10.1038/nature01055
Jones RH, Flynn KJ (2005) Nutritional status and diet composition affect the value of diatoms as copepod prey. Science 307:1457–1459. https://doi.org/10.1126/science.1107767
Kiørboe T, Tiselius P, Mitchell-Innes B, Hansen JL, Visser AW, Mari X (1998) Intensive aggregate formation with low vertical flux during an upwelling-induced diatom bloom. Limnol Oceanogr 43:104–116. https://doi.org/10.4319/lo.1998.43.1.0104
Kleppel GS, Holliday D, Pieper R (1991) Trophic interactions between copepods and microplankton: a question about the role of diatoms. Limnol Oceanogr 36:172–178. https://doi.org/10.4319/lo.1991.36.1.0172
Kranzler CF, Krause JW, Brzezinski MA, Edwards BR, Biggs WP, Maniscalco M, McCrow JP, Van Mooy BA, Bidle KD, Allen AE (2019) Silicon limitation facilitates virus infection and mortality of marine diatoms. Nat Microbiol 4:1790–1797. https://doi.org/10.1038/s41564-019-0502-x
Kuo J, Chen CY, Han CC, Ju YM, Tew KS (2021) Analyses of diet preference of larval orange-spotted grouper (Epinephelus coioides) grown under inorganic fertilization method using next-generation sequencing. Aquaculture 531:735916. https://doi.org/10.1016/j.aquaculture.2020.735916
Lebour MV (1922) The food of plankton organisms. J Mar Biolog Assoc UK 12:644–677. https://doi.org/10.1017/S0025315400009681
Lechner CC, Becker CF (2015) Silaffins in silica biomineralization and biomimetic silica precipitation. Mar Drugs 13:5297–5333. https://doi.org/10.3390/md13085297
Lee CH, Dahms HU, Cheng SH, Souissi S, Schmitt FG, Kumar R, Hwang JS (2010) Predation of Pseudodiaptomus annandalei (Copepoda: Calanoida) by the grouper fish fry Epinephelus coioides under different hydrodynamic conditions. J Exp Mar Biol Ecol 393:17–22. https://doi.org/10.1016/j.jembe.2010.06.005
Lee IS, Ohs CL, Broach JS, DiMaggio MA, Watson CA (2018) Determining live prey preferences of larval ornamental marine fish utilizing fluorescent microspheres. Aquaculture 490:125–135. https://doi.org/10.1016/j.aquaculture.2018.01.035
Leising AW, Pierson JJ, Halsband-Lenk C, Horner R, Postel J (2005) Copepod grazing during spring blooms: Does Calanus pacificus avoid harmful diatoms? Prog Oceanogr 67:384–405. https://doi.org/10.1016/j.pocean.2005.09.008
Li J, Wang Y, Liang Y, Huang J, Liu Y, Lu J (2019) Production of aldehydes in diatoms and dinoflagellates and the detrimental effect on copepod grazers. Int J Agric Biol 22:763–768. https://doi.org/10.17957/IJAB/15.1127
Liao IC, Su HM, Chang EY (2001) Techniques in finfish larviculture in Taiwan. Aquaculture 200:1–31. https://doi.org/10.1016/S0044-8486(01)00692-5
Liu GX, Xu DH (2010) Feeding, egg production and laboratory culture of Schmackeria poplesia Shen (Copepoda: Calanoida). Aquac Res 41:1817-1826. https://doi.org/10.1111/j.1365-2109.2010.02566.x
Low JS, Chew LL, Ng CC, Goh HC, Lehette P, Chong VC (2018) Heat shock response and metabolic stress in the tropical estuarine copepod Pseudodiaptomus annandalei converge at its upper thermal optimum. J Therm Biol 74:14–22. https://doi.org/10.1016/j.jtherbio.2018.02.012
Lundholm N, Krock B, John U, Skov J, Cheng J, Pančić M, Wohlrab S, Rigby K, Nielsen TG, Selander E (2018) Induction of domoic acid production in diatoms—Types of grazers and diatoms are important. Harmful Algae 79:64–73. https://doi.org/10.1016/j.hal.2018.06.005
Martin-Jézéquel V, Hildebrand M, Brzezinski MA (2000) Silicon metabolism in diatoms: implications for growth. J Phycol 36:821–840. https://doi.org/10.1046/j.1529-8817.2000.00019.x
Mendes C, Chambel J, Lopes J, Calado R, Maranhão P (2016) Effect of different diets on growth of the ciliate protozoan Euplotes sp. Front Mar Sci. Conference. https://doi.org/10.3389/conf.FMARS.2016.04.00032
Michels J, Gorb SN (2015a) Mandibular gnathobases of marine planktonic copepods–feeding tools with complex micro-and nanoscale composite architectures. Beilstein J Nanotechnol 6:674–685. https://doi.org/10.3762/bjnano.6.68
Michels J, Gorb SN (2015b) Mandibular gnathobases of marine planktonic copepods—structural and mechanical challenges for diatom frustules. In: Hamm C (eds) Evolution of lightweight structures. Springer, Dordrecht, pp 59–73. https://doi.org/10.1007/978-94-017-9398-8_4
Miller C, Nelson D, Weiss C, Soeldner A (1990) Morphogenesis of opal teeth in calanoid copepods. Mar Biol 106:91–101. https://doi.org/10.1007/BF02114678
Mitchell JG, Seuront L, Doubell MJ, Losic D, Voelcker NH, Seymour J, Lal R (2013) The role of diatom nanostructures in biasing diffusion to improve uptake in a patchy nutrient environment. PLoS One 8:e59548. https://doi.org/10.1371/journal.pone.0059548
Nogueira N, Sumares B, Andrade CAP, Afonso A (2018) The effects of temperature and photoperiod on egg hatching success, egg production and population growth of the calanoid copepod, Acartia grani (Calanoida: Acartiidae). Aquac Res 49:93–103. https://doi.org/10.1111/are.13437
Nurhidayati T, Purnobasuki H, Muslihatin W, Ferdianto A, Purwani KI (2023) The influences of N and Si on growth and lipid content of microalgae Skeletonema costatum. AIP Conference Proceedings 2554:090005. https://doi.org/10.1063/5.0106112
Ohs CL, Chang KL, Grabe SW, DiMaggio MA, Stenn E (2010) Evaluation of dietary microalgae for culture of the calanoid copepod Pseudodiaptomus pelagicus. Aquaculture 307:225–232. https://doi.org/10.1016/j.aquaculture.2010.07.016
Oliveira MD, Monteiro MPC, Robbs PG, Leite SGF (1999) Growth and chemical composition of Spirulina maxima and Spirulina platensis biomass at different temperatures. Aquac Int 7:261–275. https://doi.org/10.1023/A:1009233230706
Pai SC, Riley JP (1994) Determination of nitrate in the presence of nitrite in natural waters by flow injection analysis with a non-quantitative on-line cadmium redactor. Int J Environ Anal Chem 57:263–277. https://doi.org/10.1080/03067319408027460
Pančić M, Torres RR, Almeda R, Kiørboe T (2019) Silicified cell walls as a defensive trait in diatoms. Proc Royal Soc B 286:20190184. https://doi.org/10.1098/rspb.2019.0184
Pandey BD, Yeragi S (2004) Preliminary and mass culture experiments on a heterotrichous ciliate, Fabrea salina. Aquaculture 232:241–254. https://doi.org/10.1016/S0044-8486(03)00459-9
Parsons TR, Maita Y, Lalli CM (1984a) Determination of chlorophylls and total carotenoids: spectrophotometric method. A manual of chemical and biological methods for seawater analysis. Pergamon Press, Oxford, pp 101–104. https://doi.org/10.1016/B978-0-08-030287-4.50032-3
Parsons TR, Maita Y, Lalli CM (1984b) Determination of chlorophylls and total carotenoids: spectrophotometric method. A manual of chemical & biological methods for seawater analysis, pp 101–104
Pinto CS, LlP S-S, Santos PJP (2001) Development and population dynamics of Tisbe biminiensis (Copepoda: Harpacticoida) reared on different diets. Aquaculture 198:253–267. https://doi.org/10.1016/S0044-8486(00)00582-2
Pohnert G (2005) Diatom/copepod interactions in plankton: the indirect chemical defense of unicellular algae. ChemBioChem 6:946–959. https://doi.org/10.1002/cbic.200400348
Pondaven P, Gallinari M, Chollet S, Bucciarelli E, Sarthou G, Schultes S, Jean F (2007) Grazing-induced changes in cell wall silicification in a marine diatom. Protist 158:21–28. https://doi.org/10.1016/j.protis.2006.09.002
Rahman NA, Teh JC, Katayama T, Wahid ME, Nagao N, Yamada Y, Takahashi K (2022) Effect of newly isolated high antioxidant diatom on the reproduction and stress tolerance of the marine copepod, Acartia erythraea under crowding stress. Aquac Res 53:5365–5374. https://doi.org/10.1111/are.16019
Rasdi NW, Qin JG (2018) Impact of food type on growth, survival and reproduction of the cyclopoid copepod Cyclopina kasignete as a potential live food in aquaculture. Aquac Int 26:1281–1295. https://doi.org/10.1007/s10499-018-0283-x
Rayner TA, Jørgensen NO, Blanda E, Wu CH, Huang CC, Mortensen J, Hwang JS, Hansen BW (2015) Biochemical composition of the promising live feed tropical calanoid copepod Pseudodiaptomus annandalei (Sewell 1919) cultured in Taiwanese outdoor aquaculture ponds. Aquaculture 441:25–34. https://doi.org/10.1016/j.aquaculture.2015.01.034
Romann J, Valmalette JC, Chauton MS, Tranell G, Einarsrud MA, Vadstein O (2015) Wavelength and orientation dependent capture of light by diatom frustule nanostructures. Sci Rep 5:1–6. https://doi.org/10.1038/srep17403
Ryderheim F, Grønning J, Kiørboe T (2022) Thicker shells reduce copepod grazing on diatoms. Limnol Oceanogr Letters 7:435–442. https://doi.org/10.1002/lol2.10243
Shikata T, Nukata A, Yoshikawa S, Matsubara T, Yamasaki Y, Shimasaki Y, Oshima Y, Honjo T (2009) Effects of light quality on initiation and development of meroplanktonic diatom blooms in a eutrophic shallow sea. Mar Biol 156:875–889. https://doi.org/10.1007/s00227-009-1131-3
Sommer U, Stibor H, Katechakis A, Sommer F, Hansen T, 2002. Pelagic food web configurations at different levels of nutrient richness and their implications for the ratio fish production: primary production. In: Vadstein O, Olsen Y (eds) Sustainable increase of marine harvesting: Fundamental mechanisms and new concepts. Springer, Dordrecht, pp 11–20. https://doi.org/10.1007/978-94-017-3190-4_2
Tammilehto A, Nielsen TG, Krock B, Møller EF, Lundholm N (2015) Induction of domoic acid production in the toxic diatom Pseudo-nitzschia seriata by calanoid copepods. Aquat Toxicol 159:52–61. https://doi.org/10.1016/j.aquatox.2014.11.026
Tew KS, Meng PJ, Lin HS, Chen JH, Leu MY (2013) Experimental evaluation of inorganic fertilization in larval giant grouper (Epinephelus lanceolatus Bloch) production. Aquac Res 44:439–450. https://doi.org/10.1111/j.1365-2109.2011.03051.x
Tew KS, Chang YC, Meng PJ, Leu MY, Glover DC (2016) Towards sustainable exhibits–application of an inorganic fertilization method in coral reef fish larviculture in an aquarium. Aquac Res 47:2748–2756. https://doi.org/10.1111/are.12725
Tulsankar SS, Cole AJ, Gagnon MM, Fotedar R (2021) Temporal variations and pond age effect on plankton communities in semi-intensive freshwater marron (Cherax cainii, Austin and Ryan, 2002) earthen aquaculture ponds in Western Australia. Saudi J Biol Sci 28:1392–1400. https://doi.org/10.1016/j.sjbs.2020.11.075
Vargas CA, Escribano R, Poulet S (2006) Phytoplankton food quality determines time windows for successful zooplankton reproductive pulses. Ecology 87:2992–2999. https://doi.org/10.1890/0012-9658(2006)87[2992:PFQDTW]2.0.CO;2
Vaulot D, Olson R, Merkel S, Chisholm S (1987) Cell-cycle response to nutrient starvation in two phytoplankton species, Thalassiosira weissflogii and Hymenomonas carterae. Mar Biol 95:625–630. https://doi.org/10.1007/BF00393106
Walter TC, Ohtsuka S, Castillo LV (2006) A new species of Pseudodiaptomus (Crustacea: Copepoda: Calanoida) from the Philippines, with a key to pseudodiaptomids from the Philippines and comments on the status of the genus Schmackeria. Proc Biol Soc Wash 119:202–221. https://doi.org/10.2988/0006-324X(2006)119[202:ANSOPC]2.0.CO;2
Wang B, Xin M, Wei Q, Xie L (2018) A historical overview of coastal eutrophication in the China Seas. Mar Pollut Bull 136:394–400. https://doi.org/10.1016/j.marpolbul.2018.09.044
Wu F, Dai M, Huang H, Qi Z (2018) Plankton ciliate community responses to different aquatic environments in Nan’ao Island, a representative mariculture base in the South China Sea. Mar Freshw Res 70:426–436. https://doi.org/10.1071/MF18153
Xu H, Shi Z, Zhang X, Pang M, Pan K, Liu H (2021) Diatom frustules with different silica contents affect copepod grazing due to differences in the nanoscale mechanical properties. Limnol Oceanogr 66:3408–3420. https://doi.org/10.1002/lno.11887
Yeh HD, Questel JM, Maas KR, Bucklin A (2020) Metabarcoding analysis of regional variation in gut contents of the copepod Calanus finmarchicus in the North Atlantic Ocean. Deep Sea Res Part II Top Stud Oceanogr 180:104738. https://doi.org/10.1016/j.dsr2.2020.104738
Yin K, Qian PY, Chen JC, Hsieh DP, Harrison PJ (2000) Dynamics of nutrients and phytoplankton biomass in the Pearl River estuary and adjacent waters of Hong Kong during summer: preliminary evidence for phosphorus and silicon limitation. Mar Ecol Prog Ser 194:295–305. https://doi.org/10.3354/meps194295
Zhang A, Zhang J, Liu S, Xuan J, Zhu Z (2020) Evaluation of silicic acid sources for spring diatom blooms on the continental shelf: Insights from stable silicon isotopes in the East China Sea. J Geophys Res Oceans 125:e2019JC015478. https://doi.org/10.1029/2019JC015478
Acknowledgements
This work was supported by grants from Taiwan Ministry of Science and Technology (MOST) with grant number MOST 110-2611-M-291-001, and Taiwan National Science and Technology Council (NSTC) with grant number NSTC 111-2611-M-110-028 to KST.
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The research project was partially sponsored by Taiwan’s Ministry of Science and Technology (MOST) with grant number MOST 110–2611-M-291–001, and Taiwan’s National Science and Technology Council (NSTC) with grant number NSTC 111–2611-M-110–028 to KST.
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Guo-Kai Hong: Data curation, Formal analysis, Writing- Original draft preparation, Visualization, Investigation. Kwee Siong Tew: Conceptualization, Methodology, Supervision, Validation, Writing- Reviewing and Editing, Funding acquisition.
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Hong, GK., Tew, K.S. Assessing the effects of silicate addition on phytoplankton composition and copepod production in an inorganic fertilization system. Aquacult Int 32, 1119–1134 (2024). https://doi.org/10.1007/s10499-023-01208-2
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DOI: https://doi.org/10.1007/s10499-023-01208-2