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Prevalence, phenotypic and genotypic diversity, antibiotic resistance, and frequency of virulence genes in Pseudomonas aeruginosa isolated from shrimps

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Abstract

Preventive measures should be prioritized for the increased growth of bacterial contamination in aquatic organisms. This study is aimed at molecular investigation of the prevalence of Pseudomonas aeruginosa, antibiotic resistance, and virulence factor encoding genes. After confirmation of P. aeruginosa collected from shrimp samples (in the same size) in Isfahan and Chabahar (Iran) in summer and autumn, antibiotic resistance encoding genes, virulence factor encoding genes, and multi-drug resistance (MDR) patterns were assessed. Bacterial contamination from Isfahan was higher than Chabahar despite having a cooler climate on summer days. Antibiotic resistance to piperacillin in fresh shrimp samples in summer in Isfahan was contrary to its usage as a front-line antibiotic agent. Lowered MDR pattern in frozen shrimp viability of bacteria was related to the varied expression of antibiotic resistance, highlighting the importance of regulations for cold chain in storage, transportation, and distribution of marine samples compared to fresh shrimps. Foodborne pathogens are of clinical and environmental importance. Results of our study indicated a high rate of frequency for P. aeruginosa isolated from marine samples. Maintenance of cold chain has indispensable roles in preservation, and reduction of P. aeruginosa frequency in aquatic organisms.

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Fig. 1
Fig. 2
Fig. 3

taken from A fresh shrimp and B frozen shrimps. C Results for general comparing of frozen and fresh shrimps

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Acknowledgements

All of the authors and co-authors who meet the criteria for authorship (according to the journal’s instruction) are mentioned on the title page. All of the authors and co-authors would like to warmly appreciate Dr. Momtaz (laboratory manager of Shahrekord Branch, Islamic Azad University, Shahrekord), Mrs. S. Karimi, and Mr. M. Momeni (laboratory experts of Shahrekord Branch, Islamic Azad University, Shahrekord), for their unsparing and meticulous efforts to accomplish this study.

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Authors and Affiliations

  1. Department of Food Hygiene, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Seyed Majid Hashemi Dehkordi, Seyed Amirali Anvar & Maryam Ataee

  2. Department of Food Hygiene and Public Health, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

    Ebrahim Rahimi

  3. Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Hamed Ahari

Authors
  1. Seyed Majid Hashemi Dehkordi
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  2. Seyed Amirali Anvar
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  3. Ebrahim Rahimi
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  4. Hamed Ahari
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  5. Maryam Ataee
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Contributions

All of the authors and co-authors who were listed on the title page greatly helped to the accomplishment of this study with unsparing efforts. Hashemi Dehkordi SM helped to the preparation of the last version of the manuscript, and literature review. He also contributed to conducting an experiment, data curation, designation of images and tables, and final arrangement of the data-making structure of the study. Anvar SA mainly did project supervision and corresponding and verified the manuscript by the definitive approval for submission. He also did academic and grammatical peer revision, designation of methodological strategies, and major conceptualization qualifying. Ahari H did scientific consultations, critical revision of the manuscript for important intellectual content according to the journal's instruction, and designation of methodological strategies. Rahimi E helped with conceptualization, revision, methodology, and revision. Ataee M helped for validation of data, edition, and visualization. All of the authors and co-authors attest to the fact that they have approved the final version of the article, and they have received an electronic copy of the manuscript. Also, they attest to the validity and legitimacy of data, and agreed to submit this manuscript to this journal.

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Correspondence to Seyed Amirali Anvar.

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Dehkordi, S.M.H., Anvar, S.A., Rahimi, E. et al. Prevalence, phenotypic and genotypic diversity, antibiotic resistance, and frequency of virulence genes in Pseudomonas aeruginosa isolated from shrimps. Aquacult Int 30, 131–156 (2022). https://doi.org/10.1007/s10499-021-00798-z

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