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Influence of mannan oligosaccharide supplementation on haematological and immunological responses and disease resistance of striped catfish (Pangasianodon hypophthalmus Sauvage, 1878) juveniles

Aquaculture International volume 27, pages 1535–1551 (2019)Cite this article

Abstract

This study was carried out to evaluate the effect of mannan oligosacharide (MOS) as dietary supplement on haematological parameters, immune response and protection against Aeromonas hydrophila in striped catfish (Pangasianodon hypophthalmus, Sauvage, 1878) juveniles. Triplicate groups of juvenile striped catfish (initial weight 20.41 ± 1.64 g) were fed twice per day at 2.5% of body weight for 12 weeks, with 0 (control), 0.2, 0.4, 0.6 or 0.8% MOS diets. At the end of the feeding trial, fish were intraperitoneally challenged with 1 × 106 CFU ml−1of A. hydrophila and mortalities were recorded over 21 days. The haematological and immunological parameters were assessed both in pre- and post-challenged fish groups. Dietary inclusion of MOS did not show any significant influence on the majority of the haematological parameters of striped catfish when they were not infected. But it showed a significant positive influence on some of the most important parameters, namely RBC, WBC, lymphocyte, granulocyte and lysozyme activity and survival, after a 2-week challenge with A. hydrophila. After 2-week infection with A. hydrophila, a better concentration of red blood cell (RBC), white blood cell (WBC), granulocyte, total Ig content, lysozyme activity and survival were observed when striped catfish were fed with MOS at 0.4% and above, with significantly highest at 0.6% MOS diet than the control diet. Based on the results of this study, it is concluded that supplementation of MOS at least 0.4% is sufficient to activate the immune response and makes the striped catfish more resistant to A. hydrophila infection.

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Abbreviations

MOS:

Mannan oligosaccharide

FOS:

Fructo oligosaccharides

scFOS:

Short-chain fructo oligosaccharides

GOS:

Galacto oligosaccharides

ESR:

Erythrocyte sedimentation rate

PCV:

Packed cell volume

Hb:

Haemoglobin

RBC:

Red blood cell

WBC:

White blood cell

MCHC:

Mean corpuscular haemoglobin concentration

MCH:

Mean corpuscular haemoglobin

MCV:

Mean corpuscular volume

NaCl:

Sodium chloride

Ig:

Immunoglobulin

PEG:

Polyethylene glycol

ANOVA:

Analysis of variance

SD:

Standard deviation

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Funding

This study was financially supported by the Organization for Women in Science in the Developing World (OWSDW), Exploratory Research Grant Scheme (ERGS), the Ministry of Higher Education, Malaysia (Project No.: 203 PBIOLOGI.6730134) and the Postgraduate Research Grant Scheme in USM (PRGS) for funding this research.

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Authors and Affiliations

  1. Department of Aquaculture, Faculty of Fisheries, Hajee Mohammad Danesh Science and Technology University, Dinajpur, 5200, Bangladesh

    Mst. Nahid Akter

  2. Faculty of Science and Technology, Universiti Sains Islam Malaysia, 71800, Bandar Baru Nilai, Negeri Sembilan, Malaysia

    Roshada Hashim

  3. Faculty of Veterinary Medicine, Syiah Kuala University, Aceh, 23111, Indonesia

    Amalia Sutriana

  4. Institute of Marine Biotechnology, Universiti Malaysia Terengganu, 21030, Kuala Terengganu, Malaysia

    Siti Azizah Mohd Nor

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  1. Mst. Nahid Akter
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  2. Roshada Hashim
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  3. Amalia Sutriana
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  4. Siti Azizah Mohd Nor
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Correspondence to Mst. Nahid Akter.

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Conflict of Interest

Author A. Mst. Nahid Akter has received a grant from the Organization for Women in Science in the Developing World (OWSDW) as research fellow and a research grant from the Postgraduate Research Grant Scheme (PRGS) in USM ; author B. Roshada Hashim received a grant from the Ministry of Higher Education, Malaysia, and the Exploratory Research Grant Scheme (ERGS) as mentor.

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Akter, M.N., Hashim, R., Sutriana, A. et al. Influence of mannan oligosaccharide supplementation on haematological and immunological responses and disease resistance of striped catfish (Pangasianodon hypophthalmus Sauvage, 1878) juveniles. Aquacult Int 27, 1535–1551 (2019). https://doi.org/10.1007/s10499-019-00408-z

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Keywords

  • Immune response
  • Haematological parameters
  • Mannan oligosaccharide
  • P. hypophthalmus
  • A. hydrophila