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Astaxanthin enhances hematology, antioxidant and immunological parameters, immune-related gene expression, and disease resistance against in Channa argus

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Abstract

The present study was aimed to add 0, 50, or 100 mg/kg of astaxanthin (AST) to the basal diet of Channa argus and determine the effects on hematology, antioxidant and immunological parameters, immune-related genes expression, and disease resistance. Adding 50 or 100 mg/kg AST significantly enhanced (P < 0.05) Hb content at 14, 21, and 28 days and also the levels of Ht and RBC at 21 and 28 days. Supplementing 50 or 100 mg/kg of AST for 21 and 28 days significantly increased (P < 0.05) the level of serum antioxidant enzymes (SOD, CAT, and GSH-Px) or decreased (P < 0.05) serum MDA content. The level of serum lysozyme significantly enhanced (P < 0.05) at 28 days and also IgM and C3 at 21 days in 100 mg/kg AST treatment and at 28 days in 50 or 100 mg/kg AST treatment. Meanwhile, 50 and 100 mg/kg AST treatment significantly upregulated (P < 0.05) the expression of HSP70, HSP90, IκB-α, and GR or downregulated NF-κB p65 and inflammatory cytokines (TNF-α, IL-1β, and IL-8) transcription at 21 and 28 days. Fish fed 50 and 100 mg/kg AST enhanced (P < 0.05) disease resistance against Aeromonas hydrophila (survival rate 51.0% and 58.0%, respectively). The present results suggested that AST enhanced hematology and serum antioxidant and immune response, regulated immune-related gene expression, and increased disease resistance against A. hydrophila in C. argus.

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Acknowledgments

The research was supported by the National Natural Science Foundation of China (No. 31372540).

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Authors and Affiliations

  1. College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, Jilin, China

    Mu-Yang Li, Gui-Qin Wang & Dong-Ming Zhang

  2. Jilin Provincial Key Laboratory of Animal Nutrition and Feed Science, Jilin Agricultural University, Changchun, 130118, Jilin, China

    Mu-Yang Li, Gui-Qin Wang & Dong-Ming Zhang

  3. Xin Li Cheng Reservoir Administration Bureau of Jilin Province, Changchun, 130022, Jilin, China

    Xin-Yu Liu & Chang-Ge Xia

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  1. Mu-Yang Li
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Correspondence to Gui-Qin Wang.

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All experiments and handling of the animals were conducted according to the research protocols approved by the Institutional Animal Care and Use Committee, Jilin Agricultural University.

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Li, MY., Liu, XY., Xia, CG. et al. Astaxanthin enhances hematology, antioxidant and immunological parameters, immune-related gene expression, and disease resistance against in Channa argus. Aquacult Int 27, 735–746 (2019). https://doi.org/10.1007/s10499-019-00362-w

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