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In vitro and in vivo evaluation of probiotic properties of Enterobacter cloacae in Kenyi cichlid, Maylandia lombardoi

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Abstract

In this study, Enterobacter cloacae bacterium was isolated from curd and its antibacterial potential against the pathogen Plesiomonas shigelloides was evaluated using the freshwater ornamental fish Kenyi cichlid (Maylandia lombardoi). Among the bacterial isolates, E. cloacae exhibited tolerance to acidic pH 2 and demonstrated the highest antibacterial activity against P. shigelloides in various in vitro assays. Dietary supplementation of E. cloacae with prebiotic supplement 2% mannan oligosaccharide improved the growth performance and reduced the toxic metabolites such as nitrite in culture tank water of Kenyi cichlid. In vivo application of E. cloacae with mannan oligosaccharide significantly elevated (p < 0.05) white blood cell counts (88.47 ± 2.15 103 mm−3) and respiratory burst activity (0.243 ± 0.007) of Kenyi cichlid when challenged with P. shigelloides. Intestinal morphology of Kenyi cichlid fish treated with probiotic and prebiotic combinations showed improved intestinal architectures. The present findings confirm that the isolated bacterium E. cloacae is a potential probiotic and can be used effectively to prevent infection of P. shigelloides in freshwater ornamental fish culture.

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Acknowledgements

The authors gratefully acknowledge University Grant Commission (UGC), India, for providing grants (UGC Major Project—40-401-2011 (SR) and 04.07.2011) to pursue this work.

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  1. Disease Control and Prevention Lab, Department of Animal Health and Management, Alagappa University, Karaikudi, Tamil Nadu, 630 003, India

    Nisha Rajagopalan Girijakumari & Prabhu Narayanasamy Marimuthu

  2. Department of Zoology, Directorate of Distance Education, Alagappa University, Karaikudi, Tamil Nadu, 630 003, India

    Kannapiran Ethiraja

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  1. Nisha Rajagopalan Girijakumari
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Correspondence to Prabhu Narayanasamy Marimuthu.

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Rajagopalan Girijakumari, N., Ethiraja, K. & Narayanasamy Marimuthu, P. In vitro and in vivo evaluation of probiotic properties of Enterobacter cloacae in Kenyi cichlid, Maylandia lombardoi. Aquacult Int 26, 959–980 (2018). https://doi.org/10.1007/s10499-018-0262-2

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