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Aquaculture International

, Volume 26, Issue 4, pp 959–980 | Cite as

In vitro and in vivo evaluation of probiotic properties of Enterobacter cloacae in Kenyi cichlid, Maylandia lombardoi

  • Nisha Rajagopalan Girijakumari
  • Kannapiran Ethiraja
  • Prabhu Narayanasamy Marimuthu
Article
  • 89 Downloads

Abstract

In this study, Enterobacter cloacae bacterium was isolated from curd and its antibacterial potential against the pathogen Plesiomonas shigelloides was evaluated using the freshwater ornamental fish Kenyi cichlid (Maylandia lombardoi). Among the bacterial isolates, E. cloacae exhibited tolerance to acidic pH 2 and demonstrated the highest antibacterial activity against P. shigelloides in various in vitro assays. Dietary supplementation of E. cloacae with prebiotic supplement 2% mannan oligosaccharide improved the growth performance and reduced the toxic metabolites such as nitrite in culture tank water of Kenyi cichlid. In vivo application of E. cloacae with mannan oligosaccharide significantly elevated (p < 0.05) white blood cell counts (88.47 ± 2.15 103 mm−3) and respiratory burst activity (0.243 ± 0.007) of Kenyi cichlid when challenged with P. shigelloides. Intestinal morphology of Kenyi cichlid fish treated with probiotic and prebiotic combinations showed improved intestinal architectures. The present findings confirm that the isolated bacterium E. cloacae is a potential probiotic and can be used effectively to prevent infection of P. shigelloides in freshwater ornamental fish culture.

Keywords

E. cloacae Fish disease Kenyi cichlid Probiotics P. shigelloides 

Notes

Acknowledgements

The authors gratefully acknowledge University Grant Commission (UGC), India, for providing grants (UGC Major Project—40-401-2011 (SR) and 04.07.2011) to pursue this work.

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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Nisha Rajagopalan Girijakumari
    • 1
  • Kannapiran Ethiraja
    • 2
  • Prabhu Narayanasamy Marimuthu
    • 1
  1. 1.Disease Control and Prevention Lab, Department of Animal Health and ManagementAlagappa UniversityKaraikudiIndia
  2. 2.Department of Zoology, Directorate of Distance EducationAlagappa UniversityKaraikudiIndia

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