Skip to main content

Advertisement

SpringerLink
Log in

Short communication: gut microbiota of European sea bass (Dicentrarchus labrax) is modulated by short-chain fructooligosaccharides and xylooligosaccharides

  • Published:
Aquaculture International Aims and scope Submit manuscript

Abstract

This study aimed at assessing the effect of short-chain fructooligosaccharides (scFOS), xylooligosaccharides (XOS), and galactooligosaccharides (GOS) on European sea bass juveniles gut microbiota. Four practical diets were formulated with fish meal (FM) and plant feedstuffs (PF) as protein sources (circa 30:70 of protein from FM:PF) and to include 1% of α-cellulose (control diet, CTR), or 1% of scFOS, XOS, or GOS (diets FOS, XOS, and GOS, respectively). Triplicate groups of fish with 79 g were fed with the experimental diets during 30 days. Gut content was sampled at days 7 and 30 for allochthonous microbiota characterization. Lactobacillus, Pseudomonas, Vibrio, and Burkholderia were the main genera found in fish gut. Gut allochthonous microbiota presented an increased number of operational taxonomic units (OTUs) and a higher Margalef index of bacterial richness in fish fed scFOS and XOS. Gut allochthonous microbial OTUs, Margalef species richness index, Shannon’s diversity index, and SIMPER similarity were unaffected by sampling day. In conclusion, scFOS and XOS modulated European sea bass gut microbial community and this effect persisted throughout time. These changes in gut microbiota composition contribute to explain the positive effects on growth performance, immune status, and lipid and glucose metabolism previously reported in European sea bass fed with scFOS and XOS.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Abdelmalek BE, Driss D, Kallel F et al (2015) Effect of xylan oligosaccharides generated from corncobs on food acceptability, growth performance, haematology and immunological parameters of Dicentrarchus labrax fingerlings. Fish Physiol Biochem 41:1587–1596

    Article  CAS  PubMed  Google Scholar 

  • AOAC (2000) Official methods of analysis. Association of Official Analytical Chemists, Gaithersburg

    Google Scholar 

  • Azeredo R, Machado M, Kreuz E et al (2017) The European seabass (Dicentrarchus labrax) innate immunity and gut health are modulated by dietary plant-protein inclusion and prebiotic supplementation. Fish Shellfish Immunol 60:78–87

    Article  CAS  PubMed  Google Scholar 

  • Beutler HO (1984) Starch. In: Bergmeyer HU (ed) Methods of enzymatic analysis, vol 6. Verlag Chemie Weinheim, Basel, pp 2–10

    Google Scholar 

  • Dimitroglou A, Merrifield DL, Carnevali O et al (2011) Microbial manipulations to improve fish health and production—a Mediterranean perspective. Fish Shellfish Immunol 30:1–16

    Article  CAS  PubMed  Google Scholar 

  • Gänzle MG, Follador R (2012) Metabolism of oligosaccharides and starch in lactobacilli: a review. Front Microbiol 3:1–15

    Article  Google Scholar 

  • Geraylou Z, Souffreau C, Rurangwa E et al (2013) Prebiotic effects of arabinoxylan oligosaccharides on juvenile Siberian sturgeon (Acipenser baerii) with emphasis on the modulation of the gut microbiota using 454 pyrosequencing. FEMS Microbiol Ecol 86:357–371

    Article  CAS  PubMed  Google Scholar 

  • Ghanbari M, Kneifel W, Domig KJ (2015) A new view of the fish gut microbiome: advances from next-generation sequencing. Aquaculture 448:464–475

    Article  CAS  Google Scholar 

  • Gibson GR, Roberfroid M (1995) Dietary modulation of the human colonie microbiota: introducing the concept of prebiotics. J Nutr 125:1401–1412

    CAS  PubMed  Google Scholar 

  • Guerreiro I, Couto A, Pérez-Jiménez A et al (2015a) Gut morphology and hepatic oxidative status of European sea bass (Dicentrarchus labrax) juveniles fed plant feedstuffs or fishmeal-based diets supplemented with short-chain fructo-oligosaccharides and xylo-oligosaccharides. Br J Nutr 114:1975–1984

    Article  CAS  PubMed  Google Scholar 

  • Guerreiro I, Oliva-Teles A, Enes P (2015b) Improved glucose and lipid metabolism in European sea bass (Dicentrarchus labrax) fed short-chain fructooligosaccharides and xylooligosaccharides. Aquaculture 441:57–63

    Article  CAS  Google Scholar 

  • Guerreiro I, Enes P, Rodiles A et al (2016a) Effects of rearing temperature and dietary short-chain fructooligosaccharides supplementation on allochthonous gut microbiota, digestive enzymes activities and intestine health of turbot (Scophthalmus maximus L.) juveniles. Aquac Nutr 22:631–642

    Article  CAS  Google Scholar 

  • Guerreiro I, Serra CR, Enes P et al (2016b) Effect of short chain fructooligosaccharides (scFOS) on immunological status and gut microbiota of gilthead sea bream (Sparus aurata) reared at two temperatures. Fish Shellfish Immunol 49:122–131

    Article  CAS  PubMed  Google Scholar 

  • Guerreiro I, Oliva-Teles A, Enes P (2017a) Prebiotics as functional ingredients: focus on Mediterranean fish aquaculture. Rev Aquacult. https://doi.org/10.1111/raq.12201

  • Guerreiro I, Serra CR, Pousão-Ferreira P et al (2017b) Prebiotics effect on growth performance, hepatic intermediary metabolism, gut microbiota and digestive enzymes of white sea bream (Diplodus sargus). Aquac Nutr. https://doi.org/10.1111/anu.12543

  • Hoseinifar SH, Khalili M, Rostami HK et al (2013) Dietary galactooligosaccharide affects intestinal microbiota, stress resistance, and performance of Caspian roach (Rutilus rutilus) fry. Fish Shellfish Immunol 35:1416–1420

    Article  CAS  PubMed  Google Scholar 

  • Hoseinifar SH, Sharifian M, Vesaghi MJ et al (2014) The effects of dietary xylooligosaccharide on mucosal parameters, intestinal microbiota and morphology and growth performance of Caspian white fish (Rutilus frisii kutum) fry. Fish Shellfish Immunol 39:231–236

    Article  CAS  PubMed  Google Scholar 

  • Hoseinifar SH, Eshaghzadeh H, Vahabzadeh H et al (2016) Modulation of growth performances, survival, digestive enzyme activities and intestinal microbiota in common carp (Cyprinus carpio) larvae using short chain fructooligosaccharide. Aquac Res 47:3246–3253

    Article  CAS  Google Scholar 

  • Kühlwein H, Emery MJ, Rawling MD et al (2013) Effects of a dietary β-(1,3)(1,6)-D-glucan supplementation on intestinal microbial communities and intestinal ultrastructure of mirror carp (Cyprinus carpio L.) J Appl Microbiol 115:1091–1106

    Article  PubMed  Google Scholar 

  • Merrifield DL, Dimitroglou A, Foey A et al (2010) The current status and future focus of probiotic and prebiotic applications for salmonids. Aquaculture 302:1–18

    Article  Google Scholar 

  • Muyzer G, de Waal EC, Uitterlinden AG (1993) Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl Environ Microbiol 59:695–700

    CAS  PubMed  PubMed Central  Google Scholar 

  • Pitcher DG, Saunders NA, Owen RJ (1989) Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 8:151–156

    Article  CAS  Google Scholar 

  • Raggi T, Gatlin IIIDM (2012) Prebiotics have limited effects on nutrient digestibility of a diet based on fish meal and soybean meal in goldfish. N Am J Aquac 74:400–407

    Article  Google Scholar 

  • Ringø E, Dimitroglou A, Hoseinifar SH et al (2014) Prebiotics in finfish: an update. In: Merrifield DL, Ringø E (eds) Aquaculture nutrition: gut health, probiotics and prebiotics. John Wiley & Sons, Ltd, Chichester, pp 360–400

    Google Scholar 

  • Romero J, Ringø E, Merrifield DL (2014) The gut microbiota of fish. In: Merrifield DL, Ringø E (eds) Aquaculture nutrition: gut health, probiotics and prebiotics. John Wiley & Sons, Ltd, Chichester, pp 75–100

    Google Scholar 

  • Zhou Q-C, Buentello JA, Gatlin DM (2010) Effects of dietary prebiotics on growth performance, immune response and intestinal morphology of red drum (Sciaenops ocellatus). Aquaculture 309:253–257

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We would like to express our thanks to P. Correia for the assistance during the trial. Authors would also thank Jefo Species-specific additives France, for providing the scFOS.

Funding

This work was partially funded by Projects AQUAIMPROV (reference NORTE-07-0124-FEDER-000038) and Pest-C/MAR/LA0015/2013 and UID/Multi/04423/2013, co-financed by the North Portugal Regional Operational Programme (ON.2-O Novo Norte), under the National Strategic Reference Framework (NSRF), through the European Regional Development Fund (ERDF), and through the COMPETE-Operational Competitiveness Programme and national funds through FCT-Foundation for Science and Technology, respectively. I. Guerreiro and P. Enes were supported by FCT grants (SFRH/BD/76139/2011 and BPD/39688/2007, respectively). C. Serra was recipient of a grant within the Project AQUAIMPROV (NORTE-07-0124-FEDER-000038).

Author information

Authors and Affiliations

  1. CIMAR/CIIMAR-Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos s/n, 4450-208, Matosinhos, Portugal

    Inês Guerreiro, Cláudia R. Serra, Aires Oliva-Teles & Paula Enes

  2. Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre s/n, Ed. FC4, 4169-007, Porto, Portugal

    Inês Guerreiro & Aires Oliva-Teles

Authors
  1. Inês Guerreiro
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Cláudia R. Serra
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Aires Oliva-Teles
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Paula Enes
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Inês Guerreiro.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Guerreiro, I., Serra, C.R., Oliva-Teles, A. et al. Short communication: gut microbiota of European sea bass (Dicentrarchus labrax) is modulated by short-chain fructooligosaccharides and xylooligosaccharides. Aquacult Int 26, 279–288 (2018). https://doi.org/10.1007/s10499-017-0220-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10499-017-0220-4

Keywords

Search

Navigation