Diet supplemented with Grifola gargal mushroom enhances growth, lipid content, and nutrient retention of juvenile rainbow trout (Oncorhynchus mykiss)
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This study examined the suitability of the edible mushroom Grifola gargal as a dietary supplement for juvenile rainbow trout (Oncorhynchus mykiss). Three treatments were established in triplicate using 50 fish (0.33 ± 0.01 g) held in 50-L containers. Treatments consisted of feeds (42–45% protein, ca. 18% lipid) supplemented with fruiting-bodies of G. gargal at 0 g kg−1 (control diet (CTRL)), 25 g kg−1 (GG25), or 100 g kg−1 (GG100). Fish were hand-fed to apparent satiation twice a day (except on Sundays) for 56 days. Feed intake and growth were recorded throughout the study, and fish body proximate composition and nutrient retention were assessed at the end of the trial. Fish given GG25 diet had better growth and feed utilization than those given the other feeds. Final body weight was 2.37 ± 0.04 g (CTRL), 4.07 ± 0.07 g (GG25), and 1.94 ± 0.06 g (GG100) and the thermal-unit growth coefficient increased significantly from 0.64 ± 0.01 in CTRL to 0.87 ± 0.01 in GG25. The feed efficiency and the protein efficiency ratio were best for fish fed GG25, and body lipid was 42.3 ± 2.6 g kg−1 in CTRL and 75.3 ± 1.5 g kg−1 in GG25 treatments. This coincided with a lower viscerosomatic index in the fish given GG25 than in those provided with the other feeds. These results suggest that dietary supplementation with G. gargal at 25 g kg−1 enhances growth and leads to improved feed utilization in small rainbow trout.
KeywordsEdible mushrooms Feed ingredients Fungi Nutrition Salmonids
This study was supported by the grants CONICET PIP 11220130100529 and ANPCYT PICT 2013-1415 to CML. We also acknowledge funding by Ministerio de Seguridad, Trabajo y Ambiente de la Provincia de Neuquén. The authors wish to thank Sofia Mc Cabe, Julián Simon and Jorge Biorkman for their contributions to this work. We acknowledge two anonymous reviewers for their constructive suggestions.
Compliance with ethical standards
The experimental protocols were approved by the Bioethics Committee, School of Biochemical and Pharmaceutical Sciences, National University of Rosario, Argentina (6060/116).
- AOAC (1990) Official methods of analysis of AOAC, 15th edn. AOAC International, ArlingtonGoogle Scholar
- De Bruijn J, Loyola C, Aqueveque P et al (2008) Influence of heat treatment on the antioxidant properties of Grifola gargal hydro-alcoholic extracts. Micol Apl Int 1:27–34Google Scholar
- De Bruijn J, Loyola C, Aqueveque P et al (2009) Antioxidant properties of extracts obtained from Grifola gargal mushrooms. Micol Apl Int 21:11–18Google Scholar
- Dobsíková R, Blahová J, Mikulíková I et al (2013) The effect of oyster mushroom β-1.3/1.6-D-glucan and oxytetracycline antibiotic on biometrical, haematological, biochemical, and immunological indices, and histopathological changes in common carp (Cyprinus carpio L). Fish Shellfish Immun 35:1813–1823CrossRefGoogle Scholar
- FAO (2012) The state of world fisheries and aquaculture. Food and Agriculture Organization of the United Nations, Rome, p 209Google Scholar
- FAO (2016) The state of world fisheries and aquaculture. Food and Agriculture Organization of the United Nations, Rome, p 200Google Scholar
- Harada E, D’Alessandro-Gabazza CN, Toda M et al (2015a) Amelioration of atherosclerosis by the new medicinal mushroom Grifola gargal Singer. J of med food 00:1–10Google Scholar
- Katya K, Yun Y, Park G et al (2014) Evaluation of the efficacy of fermented by-product of mushroom, Pleurotus ostreatus, as a fish meal replacer in juvenile Amur catfish, Silurus asotus: effects on growth, serological characteristics and immune responses. Asian Austral J Anim Sci 27:1478–1486CrossRefGoogle Scholar
- Mostak A, Abdullah N, Shuib A et al (2015) Improvement of growth and antioxidant status in Nile tilapia, Oreochromis niloticus, fed diets supplemented with mushroom stalk waste hot water extract. Aquac Res 48:1146–1157Google Scholar
- NRC (National Research Council) (1993) Nutrient requirements of fish. The National Academies Press, Washington DCGoogle Scholar
- Paripuranam T, Divya V, Ulaganathan P et al (2011) Replacing fish meal with earthworm and mushroom meals in practical diets of Labeo rohita and Hemigrammus caudovittatus fingerlings. Indian J Anim Res 45:115–119Google Scholar
- Rajchenberg M (2002) The genus Grifola (Aphyllophorales, Basidiomycota) in Argentina revisited. Bol Soc Argent Bot 37:19–27Google Scholar
- Stamets P, Zwickey H (2014) Medicinal mushrooms: ancient remedies meet modern science. Integrative Med Clin J 13:46–47Google Scholar
- Wasser SP (2014) Medicinal mushroom science: current perspectives, advances, evidences, and challenges. Biom J 37:354–356Google Scholar
- Woynarovich A, Hoitsy G, Moth-Poulsen T (2011) Small-scale rainbow trout farming. FAO Fisheries and Aquaculture Technical Paper No. 561. Food and Agriculture Organization of the United Nations, Rome, p 81Google Scholar
- Zar JH (1999) Biostatistical analysis. Prentice Hall, Upper Saddle RiverGoogle Scholar