Aquaculture International

, Volume 25, Issue 3, pp 1107–1122 | Cite as

Effect of Cynodon dactylon extract on white spot virus-infected Litopenaeus vannamei

  • O. Tomazelli Junior
  • F. Kuhn
  • P. J. Mendonça Padilha
  • L. R. Mota Vicente
  • S. Winckler da Costa
  • B. Corrêa da Silva
  • D. Dias Schleder
  • A. A. Boligon
  • J. Scapinello
  • C. Nunes Nesi
  • J. Dal Magro
  • S. De Lamo CastellvíEmail author


The objectives of this research were to study the phytochemical composition of Cynodon dactylon and investigate if the oral administration of this plant extract adsorbed on the pellet feed was capable of protecting L. vannamei challenged with white spot syndrome virus (WSSV). The shrimps were distributed into five batches, and experiments were run by triplicate: uninfected shrimps fed with pelleted feed without ethanolic extract of C. dactylon (ECDE, TC), infected shrimps fed with pelleted feed without ECDE (T1), infected shrimps fed with 1% of ECDE (T2), infected shrimps fed with 2% of ECDE (T3), and infected shrimps fed with 4% of ECDE (T4). The phytochemical screening of ECDE showed several compounds such as with important biological activities consistent with the results observed in vivo. Treatment with 2% ECDE showed a protective effect against WSSV and survival of 62% with no clinical signs of infection.


Cynodon dactylon Litopenaeus vannamei White spot virus Phytochemical screening Phenoloxidase Total hemocytes Lectins 



The authors would like to acknowledge FINEP-Recarcina and Departament d’Enginyeria Química from Universitat Rovira i Virgili for their financial support. This study is in memoriam of Margareth Linde Athayde.


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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  • O. Tomazelli Junior
    • 1
  • F. Kuhn
    • 2
  • P. J. Mendonça Padilha
    • 3
  • L. R. Mota Vicente
    • 3
  • S. Winckler da Costa
    • 3
  • B. Corrêa da Silva
    • 3
  • D. Dias Schleder
    • 4
  • A. A. Boligon
    • 5
  • J. Scapinello
    • 2
  • C. Nunes Nesi
    • 6
  • J. Dal Magro
    • 2
  • S. De Lamo Castellví
    • 1
    Email author
  1. 1.Departament d’Enginyeria QuímicaUniversitat Rovira i VirgiliTarragonaSpain
  2. 2.Ciências AmbientaisUniversidade Comunitária da Região de Chapecó—UnochapecóChapecóBrazil
  3. 3.Epagri, Centro de Desenvolvimento da Aquicultura e PescaFlorianópolisBrazil
  4. 4.Instituto Federal de Santa Catarina (IFSC)AraquariBrazil
  5. 5.Laboratório de Fitoquímica, Departamento de Farmácia IndustrialUniversidade Federal de Santa Maria (UFSM)Santa MariaBrazil
  6. 6.Epagri–Centro de Pesquisa para Agricultura Familiar (Cepaf)ChapecóBrazil

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