Aquaculture International

, Volume 25, Issue 2, pp 837–848 | Cite as

Effect of dietary fructooligosaccharide supplementation on growth, body composition, hematological and immunological parameters of Asian seabass (Lates calcarifer)

  • Sajjad Syed Raffic Ali
  • Kondusamy AmbasankarEmail author
  • Peter Ezhil Praveena
  • Sambasivam Nandakumar
  • Jagabatula Syamadayal


A feeding trial to study the effect of addition of prebiotic fructooligosaccharide (FOS) on feeds for juvenile Asian seabass (Lates calcarifer) (12.2 ± 0.4 g) was carried out. Five isonitrogenous and isocaloric experimental diets were formulated that contained 400 g kg−1 protein and 90 g kg−1 lipid. The FOS was supplemented at 2.5, 5, 7.5 and 10 g kg−1 in the diet of L. calcarifer with a control that was devoid of FOS. The trial was carried out in 1000-L fiber reinforced polymer (FRP) tanks with three replicates (each containing 20 fish) for each treatment. After 45 days of feeding, it was observed that FOS supplementation at 10 g kg−1 in the diet resulted in significantly (P < 0.05) higher final biomass (334.2 ± 7.3 g) and survival (97.7 ± 3.8 %). There were no significant (P > 0.05) differences in biological indices of fish fed with the experimental and control diets. Whole body chemical composition of animals post-feeding revealed significantly (P < 0.05) higher crude protein, crude lipid and total ash content in 5 g kg−1 FOS-supplemented diet. The analysis of hematological parameters revealed that red blood cells (RBC), white blood cells, hemoglobin, packed cell volume, mean corpuscular volume, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration levels increased significantly (P < 0.05) with dietary FOS supplementation up to 5 g kg−1.The second-degree polynomial regression analysis of RBC (4.06 ± 0.05 × 106 mm−3) showed that FOS 5 g kg−1 is an optimal level in seabass diet. Significantly (P < 0.05) higher lysozyme (77.4 ± 0.5 U mL−1) and superoxide dismutase (75.2 ± 0.3 U mL−1) activity was recorded at 10 g kg−1 FOS supplementation. It could therefore be concluded that 10 g kg−1 FOS supplementation has a beneficial effect in improving the survival rate and immunological parameters in L. calcarifer juveniles.


Barramundi Biological indices Feed additive Prebiotics Survival 



The authors are grateful to the Indian Council of Agricultural Research, New Delhi, for the project on Outreach Activity on Fish feeds. Authors express their sincere thanks to Dr. A.G. Ponniah, Former Director, and Dr. K.K. Vijayan, Director, Central Institute of Brackishwater Aquaculture, for providing necessary facilities for carrying out this work. We are thankful to Dr. G. Gopikrishna, Head, Nutrition Genetics and Biotechnology Division of CIBA, for his help in preparation of this manuscript.


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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  • Sajjad Syed Raffic Ali
    • 1
  • Kondusamy Ambasankar
    • 1
    Email author
  • Peter Ezhil Praveena
    • 1
  • Sambasivam Nandakumar
    • 1
  • Jagabatula Syamadayal
    • 1
  1. 1.Nutrition Genetics and Biotechnology DivisionCentral Institute of Brackishwater Aquaculture (ICAR)ChennaiIndia

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