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Dietary supplementation with mannan oligosaccharide influences growth, digestive enzymes, gut morphology, and microbiota in juvenile striped catfish, Pangasianodon hypophthalmus

Abstract

This study was conducted to evaluate the effects of mannan oligosaccharide (MOS) dietary supplementation on growth, digestive enzyme activities, gut morphology, and microbiota in striped catfish (Pangasianodon hypophthalmus). Triplicate groups of juvenile striped catfish (initial weight 20.41 ± 1.64 g) were fed twice per day at 2.5 % of body weight for 12 weeks, with 0 (control), 0.2, 0.4, 0.6, or 0.8 % MOS diets. Compared to control (0.74 ± 0.03) and fish fed low MOS concentration, those fed 0.6 % (1.01 ± 0.02) or 0.8 % (1.03 ± 0.03) MOS had significantly higher (P < 0.05) specific growth rates. Feed conversion ratio and protein efficiency ratio, respectively, significantly improved (P < 0.05) in fish fed 0.6 % (1.62 ± 0.23; 1.71 ± 0.23) or 0.8 % (1.42 ± 0.06; 1.92 ± 0.08) MOS. Further, compared to control and fish fed 0.2 % MOS, those fed 0.6 % MOS had significantly higher (P < 0.05) apparent protein and dry matter digestibilities. Amylase, protease, and lipase activities, respectively, were significantly higher (P < 0.05) in fish fed 0.6 % MOS (5.10 ± 0.97; 0.47 ± 0.08; and 5.59 ± 1.82) than in control (2.60 ± 0.53; 0.32 ± 0.05; and 3.78 ± 0.72). Histological analysis of the anterior and posterior gut showed significantly higher (P < 0.05) villus length in 0.6 and 0.8 % MOS fed fish than in the control and other treatments. The guts of fish fed different MOS concentrations showed similar total lactic acid bacteria counts, which were significantly higher (P < 0.05) than those in the control. Thus, 0.6 % MOS-supplemented diet improved growth, feed utilization, digestive enzyme activities, gut morphology, and microbiota in juvenile striped catfish.

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Abbreviations

MOS:

Mannan oligosaccharide

CMC:

Carboxymethyl cellulose

NFE:

Nitrogen-free extract

GE:

Gross energy

HSI:

Hepatosomatic index

IPF:

Intraperitoneal fat

VSI:

Viscerosomatic index

SGR:

Specific growth rate

FCR:

Food conversion ratio

PER:

Protein efficiency ratio

AOAC:

Association of official analytical chemists

PVA:

Polyvinyl alcohol

TEM:

Transmission electron microscope

LAB:

Lactic acid bacteria

TB:

Total bacteria

APD:

Apparent protein digestibility

ADM:

Apparent dry matter digestibility

CFU:

Colony-forming unit

MRS:

De Man, Rogosa, and Sharpe

TSA:

Tryptic soy agar

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Acknowledgments

This research was supported by the Organization for Women in Science in the Developing World and Postgraduate Research Grant Scheme in USM.

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Correspondence to Mst. Nahid Akter.

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Akter, M.N., Sutriana, A., Talpur, A.D. et al. Dietary supplementation with mannan oligosaccharide influences growth, digestive enzymes, gut morphology, and microbiota in juvenile striped catfish, Pangasianodon hypophthalmus . Aquacult Int 24, 127–144 (2016). https://doi.org/10.1007/s10499-015-9913-8

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Keywords

  • Digestive enzyme activities
  • Growth performance
  • Gut microbiota
  • Intestinal structure
  • Nutrient digestibility
  • Pangasianodon hypophthalmus
  • Prebiotics