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Growth enhancement of rainbow trout (Oncorhynchus mykiss) by passive immunization against somatostatin-14

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Abstract

Juvenile rainbow trout (Oncorhynchus mykiss) were passively immunized by intraperitoneal immunization against somatostatin-14 (SS-14) using an antibody originating from egg-laying chicken (Gallus domesticus). Fish were immunized weekly (0, 7, 14, 21, 28, 35 days) with chicken egg yolk-derived immunoglobulin (IgY) against SS-14 (1:25 IgY, 5 mg mL−1), and growth performance, feed utilization as well as plasma concentrations and mRNA levels of growth hormone (GH) and insulin-like growth factor I (IGF-I) were compared to the control group that received placebo immunization with PBS. Passive immunization significantly increased weight gain of treated fish (67.7 ± 7.4 g) compared to the control group (40.1 ± 2.0 g) after 35 days (p < 0.05). Feed conversion ratio (FCR) was significantly improved in the immunized fish (0.7 ± 0.08) compared to control group (1.2 ± 0.06) (p < 0.05). The concentrations of GH and IGF-I in the blood plasma showed no significant differences between the fish treated with anti-SS-14 and those of control during the treatment (p > 0.05). In both groups, GH levels decreased over the 35 days of the experiment (p < 0.05). However, IGF-I level during the period of treatment remained unchanged in both control and immunized fish with the anti-SS-14. Similarly, no changes were observed in pituitary GH and liver IGF-I mRNA levels between treatment and control at each sampling time (p > 0.05). There was no indication of a cumulative, long-lasting effect of repeated immunization on GH or IGF-I plasma concentrations or mRNA expression. The present study shows that a passive immunization of rainbow trout against SS-14 using a chicken egg yolk-derived SS-14 antibody could increase growth rate and improved FCR.

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References

  • Agustsson T, Björnsson BTh (2000) Growth hormone inhibits growth hormone secretion from the rainbow trout pituitary in vitro. Comp Biochem Physiol C 126:299–303

    CAS  Google Scholar 

  • Blaise O, Weil C, Le Bail PY (1995) Role of IGF-I in the control of GH secretion in rainbow trout (Oncorhynchus mykiss). Growth Regul 5:142–150

    PubMed  CAS  Google Scholar 

  • Brazeau P, Vale W, Burgus R, Ling N, Butcher M, Rivier J, Guillemi R (1973) Hypothalamic polypeptide that inhibits secretion of immunoreactive pituitary growth hormone. Science 179:77–79

    Article  PubMed  CAS  Google Scholar 

  • Canosa LF, Chang JP, Peter RE (2007) Neuroendocrine control of growth hormone in fish. Gen Comp Endocrinol 151:1–26

    Article  PubMed  CAS  Google Scholar 

  • Conlon JM, Tostivint H, Vaudry H (1997) Somatostatin- and urotensin II-related peptides: molecular diversity and evolutionary perspectives. Regul Pept 69:95–103

    Article  PubMed  CAS  Google Scholar 

  • da Silva WD, Tambourgi DV (2010) IgY: a promising antibody for use in immunodiagnostic and in immunotherapy. Vet Immunol Immunopathol 135:173–180

    Article  Google Scholar 

  • Diez JM, Giannico G, Mclean E, Donaldson EM (1992) The effect of somatostatins (Srif-14, Srif-25 and Srif-28), Galanin and Anti-Srif on Plasma Growth-Hormone Levels in Coho Salmon (Oncorhynchus kisutch, Walbaum). J Fish Biol 40:887–893

    Article  CAS  Google Scholar 

  • Drennon K, Moriyama S, Kawauchi H, Small B, Silverstein J, Parhar I, Shepherd B (2003) Development of an enzyme-linked immunosorbent assay for the measurement of plasma growth hormone (GH) levels in channel catfish (Ictalurus punctatus): assessment of environmental salinity and GH secretogogues on plasma GH levels. Gen Comp Endocrinol 133:314–322

    Article  PubMed  CAS  Google Scholar 

  • Eppler E, Caelers A, Shved N, Hwang G, Rahman AM, Maclean N, Zapf J, Reinecke M (2007) Insulin-like growth factor I (IGF-I) in a growth-enhanced transgenic (GH-overexpressing) bony fish, the tilapia (Oreochromis niloticus): indication for a higher impact of autocrine/paracrine than of endocrine IGF-I. Transgenic Res 16:479–489

    Article  PubMed  CAS  Google Scholar 

  • Fleige S, Pfaffl MW (2006) RNA integrity and the effect on the real-time qRT-PCR performance. Mol Asp Med 27:126–139

    Article  CAS  Google Scholar 

  • Gahete MD, Cordoba-Chacon J, Duran-Prado M, Malagon MM, Martinez-Fuentes AJ, Gracia-Navarro F, Luque RM, Castano JP (2010) Somatostatin and its receptors from fish to mammals. In: Vaudry H, Shioda S (eds) Phylogenetic aspects of neuropeptides, vol 1200. Annals of the NY Academy of Science, New York, pp 43–52

    Google Scholar 

  • Kroeckel S, Harjes AGE, Roth I, Katz H, Wuertz S, Susenbeth A, Schulz C (2012) When a turbot catches a fly: evaluation of a pre-pupae meal of the Black soldier fly (Hermetia illucens) as fish meal substitute—growth performance and chitin degradation in juvenile turbot (Psetta maxima). Aquaculture 364:345–352

    Article  Google Scholar 

  • Lakeh AAB, Farahmand H, Mirvaghefi A, Kloas W, Peterson BC, Wuertz S (2011) GH and IGF-I induction by passive immunisation of rainbow trout Oncorhynchus mykiss (Walbaum) using a somatostatin-14 antibody. Aquaculture 316:99–103

    Article  CAS  Google Scholar 

  • Lin XW, Peter RE (2001) Somatostatins and their receptors in fish. Comp Biochem Physiol B 129:543–550

    Article  PubMed  CAS  Google Scholar 

  • Martı-Palanca H, Martınez-Barbera JP, Pendon C, Valdivia MM, Perez-Sanchez J, Kaushik S (1996) Growth hormone as a function of age and dietary protein:energy ratio in a marine teleost, the gilthead sea bream (Sparus aurata). Growth Regul 6:253–259

    PubMed  Google Scholar 

  • Mayer I, Mclean E, Kieffer TJ, Souza LM, Donaldson EM (1994) Antisomatostatin induced growth acceleration in Chinook salmon (Oncorhynchus tshawytscha). Fish Physiol Biochem 13:295–300

    Article  PubMed  CAS  Google Scholar 

  • Nelson LE, Sheridan MA (2005) Regulation of somatostatins and their receptors in fish. Gen Comp Endocrinol 142:117–133

    Article  PubMed  CAS  Google Scholar 

  • Opitz R, Lutz I, Nguyen NH, Scanlan TS, Kloas W (2006) Analysis of thyroid hormone receptor beta A mRNA expression in Xenopus laevis tadpoles as a means to detect agonism and antagonism of thyroid hormone action. Toxicol Appl Pharmacol 212:1–13

    Article  PubMed  CAS  Google Scholar 

  • Patel YC (1999) Somatostatin and its receptor family. Front Neuroendocrinol 20:157–198

    Article  PubMed  CAS  Google Scholar 

  • Pérez Sánchez J, Weil C, Bail PY (1992) Effects of human insulin-like growth factor-I on release of growth hormone by rainbow trout (Oncorhynchus mykiss) pituitary cells. J Exp Zool 262:287–290

    Article  PubMed  Google Scholar 

  • Peterson BC, Simpson PR, Cain KD, Hardy RH, Schelling GT, Ott TL (2003) Effects of administration of somatostatin-14 and immunoneutralization of somatostatin on endocrine and growth responses in rainbow trout. J Fish Biol 63:506–522

    Article  CAS  Google Scholar 

  • Pfaffl MW (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29:2002–2007

    Article  Google Scholar 

  • Raven PA, Sakhrani D, Beckman B, Neregard L, Sundstrom LF, Bjornsson BT, Devlin RH (2012) Growth and endocrine effects of recombinant bovine growth hormone treatment in non-transgenic and growth hormone transgenic coho salmon. Gen Comp Endocrinol 177:143–152

    Article  PubMed  CAS  Google Scholar 

  • Reindl KM, Sheridan MA (2012) Peripheral regulation of the growth hormone-insulin-like growth factor system in fish and other vertebrates. Comp Biochem Physiol A 163:231–245

    Article  CAS  Google Scholar 

  • Reinecke M, Bjornsson BT, Dickhoff WW, McCormick SD, Navarro I, Power DM, Gutierrez J (2005) Growth hormone and insulin-like growth factors in fish: where we are and where to go. Gen Comp Endocrinol 142:20–24

    Article  PubMed  CAS  Google Scholar 

  • Rose ME, Orlans E, Buttress N (1974) Immunoglobulin classes in hens egg: their segregation in yolk and white. Eur J Immunol 4:521–523

    Article  PubMed  CAS  Google Scholar 

  • Sheridan MA, Hagemeister AL (2010) Somatostatin and somatostatin receptors in fish growth. Gen Comp Endocrinol 167:360–365

    Article  PubMed  CAS  Google Scholar 

  • Sheridan MA, Eilertson CD, Kerstetter TH (1998) Changes in plasma somatostatin associated with seawater adaptation and stunting of coho salmon, Oncorhynchus kisutch. Aquaculture 168:195–203

    Article  CAS  Google Scholar 

  • Sheridan MA, Kittilson JD, Slagter BJ (2000) Structure-function relationships of the signaling system for the somatostatin peptide hormone family. Am Zool 40:269–286

    CAS  Google Scholar 

  • Slagter BJ, Sheridan MA (2004) Differential expression of two somatostatin receptor subtype 1 mRNAs in rainbow trout (Oncorhynchus mykiss). J Mol Endocrinol 32:165–177

    Article  PubMed  CAS  Google Scholar 

  • Slawski H, Adem H, Tressel RP, Wysujack K, Koops U, Wuertz S, Schulz C (2011) Replacement of fish meal with rapeseed protein concentrate in diets fed to wels catfish (Silurus glanis L.). Aquac Nutr 17:605–612

    Article  CAS  Google Scholar 

  • Small BC, Peterson BC (2005) Establishment of a time-resolved fluoroimmunoassay for measuring plasma insulin-like growth factor I (IGF-I) in fish: effect of fasting on plasma concentrations and tissue mRNA expression of IGF-I and growth hormone (GH) in channel catfish (Ictalurus punctatus). Domest Anim Endocrinol 28:202–215

    Article  PubMed  CAS  Google Scholar 

  • Trubiroha A, Kroupova H, Wuertz S, Frank SN, Sures B, Kloas W (2010) Naturally-induced endocrine disruption by the parasite Ligula intestinalis (Cestoda) in roach (Rutilus rutilus). Gen Comp Endocrinol 166:234–240

    Article  PubMed  CAS  Google Scholar 

  • Tusche K, Wuertz S, Susenbeth A, Schulz C (2011a) Feeding fish according to organic aquaculture guidelines EC 710/2009: influence of potato protein concentrates containing various glycoalkaloid levels on health status and growth performance of rainbow trout (Oncorhynchus mykiss). Aquaculture 319:122–131

    Article  CAS  Google Scholar 

  • Tusche K, Berends K, Wuertz S, Susenbeth A, Schulz C (2011b) Evaluation of feed attractants in potato protein concentrate based diets for rainbow trout (Oncorhynchus mykiss). Aquaculture 321:54–60

    Article  CAS  Google Scholar 

  • Very NM, Sheridan MA (2002) The role of somatostatins in the regulation of growth in fish. Fish Physiol Biochem 27:217–226

    Article  CAS  Google Scholar 

  • Very NM, Sheridan MA (2007) Somatostatin regulates hepatic growth hormone sensitivity by internalizing growth hormone receptors and by decreasing transcription of growth hormone receptor mRNAs. Am J Physiol 292:R1956–R1962

    CAS  Google Scholar 

  • Very NM, Knutson D, Kittilson JD, Sheridan MA (2001) Somatostatin inhibits growth of rainbow trout. J Fish Biol 59:157–165

    Article  CAS  Google Scholar 

  • Very NM, Kittilson JD, Klein SE, Sheridan MA (2008) Somatostatin inhibits basal and growth hormone-stimulated hepatic insulin-like growth factor-I production. Mol Cell Endocrinol 281:19–26

    Article  PubMed  CAS  Google Scholar 

  • Wuertz S, Gessner J, Kirschbaum F, Kloas W (2007) Expression of IGF-I and IGF-I receptor in male and female sterlet, Acipenser ruthenus—evidence for an important role in gonad maturation. Comp Biochem Physiol A 147:223–230

    Article  CAS  Google Scholar 

  • Xu YP, Li XY, Jin LJ, Zhen YH, Lu YN, Li SY, You JS, Wang LH (2011) Application of chicken egg yolk immunoglobulins in the control of terrestrial and aquatic animal diseases: a review. Biotechnol Adv 29:860–868

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

The authors thank Peter Löschau for technical assistance during blood and tissue sampling, PCR and sequencing reactions.

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Authors and Affiliations

  1. Department of Ecophysiology and Aquaculture, Leibniz- Institute of Freshwater Ecology and Inland Fisheries, Müggelseedamm 310, 12587, Berlin, Germany

    Amir Abbas Bazyar Lakeh, Werner Kloas, Achim Trubiroha & Sven Wuertz

  2. Faculty of Life Sciences, Albrecht Daniel Thaer-Institute of Agricultural and Horticultural Sciences, Humboldt University of Berlin, Invaliden Str. 42, 10115, Berlin, Germany

    Amir Abbas Bazyar Lakeh & Sven Wuertz

  3. Department of Endocrinology, Faculty of Life Sciences, Institute of Biology, Humboldt University of Berlin, Invaliden Str. 42, 10115, Berlin, Germany

    Werner Kloas

  4. Department of Fishery and Environment, Faculty of Natural Resources, University College of Agriculture and Natural Resources, University of Tehran, P.O. Box: 31585–4314, Karaj, Iran

    Hamid Farahmand & Alireza Mirvaghefi

  5. Warmwater Aquaculture Research Unit, USDA, P.O. Box 38, Stoneville, MS, 38776, USA

    Brian C. Peterson

Authors
  1. Amir Abbas Bazyar Lakeh
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  2. Hamid Farahmand
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  3. Werner Kloas
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  4. Alireza Mirvaghefi
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  5. Achim Trubiroha
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  6. Brian C. Peterson
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  7. Sven Wuertz
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Correspondence to Amir Abbas Bazyar Lakeh.

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Bazyar Lakeh, A.A., Farahmand, H., Kloas, W. et al. Growth enhancement of rainbow trout (Oncorhynchus mykiss) by passive immunization against somatostatin-14. Aquacult Int 24, 11–21 (2016). https://doi.org/10.1007/s10499-015-9905-8

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  • DOI: https://doi.org/10.1007/s10499-015-9905-8

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