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Impact of season and grafter skill on nucleus retention and pearl oyster mortality rate in Pinctada margaritifera aquaculture

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Abstract

The mollusc Pinctada margaritifera is the top economic aquaculture species in French Polynesia (export value) and forms the basis of the black pearl industry. Mass production of this unique gem, produced by a living organism, relies on a surgical operation requiring tissue from a donor pearl oyster to be grafted, together with a nucleus made of shell, into the gonad of a receiving pearl oyster. This technique is performed by expert grafters, whose work constitutes the first step influencing pearl farm production yield. This study makes the first report of effects mediated by individual grafters and by the season of grafting on three rates scored at 45 days post operation: nucleus retention, nucleus rejection and pearl oyster mortality. These results were obtained in a very large scale grafting experiment, designed and conducted in a single culture site in the atoll of Arutua (Tuamotu Archipelago) and involving a total of 52,910 grafts performed by ten professional grafters, during two contrasting seasons: autumn (four grafting experiments) and spring (three grafting experiments). Statistical analysis using linear mixed-effect model for both univariate and multivariate analysis was performed. The results show a very highly significant season effect (p < 0.001), with a higher rate of nucleus retention in autumn than spring: 91.1 versus 79.4 %, respectively. A very highly significant season effect was also recorded for nucleus rejection rate (p < 0.001), which was greater in spring than in autumn: 12.6 versus 7.7 %. Oyster mortality rate was up to six times higher in spring than in autumn (p < 0.001). Within the two seasons, there was no significant difference between the grafting experiments for any of the three variables, adding robustness to the inter-season results. Taking into account this seasonal variation effect, grafter skill had a significant impact on the three variables (p < 0.001), making it possible to rank grafters from most to least efficient. Aside from skill differences between grafters, grafting in autumn may significantly increase graft success. Water temperature and its indirect consequences could be one of the most important environmental factors implicated in overall efficiency. These findings may help the potential development of an annual grafting schedule according to season/ lagoon temperature as a way to maximize production yield for the black-lipped pearl oyster industry in French Polynesia.

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References

  • Acosta-Salmón H, Martínez-Fernández E, Southgate PC (2005) Use of relaxants to obtain saibo tissue from the blacklip pearl oyster (Pinctada margaritifera) and the Akoya pearl oyster (Pinctada fucata). Aquaculture 246:167–172

    Article  Google Scholar 

  • Alagarswami K (1970) Pearl culture in Japan and its lessons for India. In: Proceedings of the Symposium on Mollusca Part III. Mar Biol Assoc India 975–998

  • Arnaud-Haond S, Bonhomme F, Blanc F (2003) Large discrepancies in differentiation of allozymes, nuclear and mitochondrial DNA loci in recently founded Pacific populations of the pearl oyster Pinctada margaritifera. J Evolutionary Biol 16:388–398

    Article  CAS  Google Scholar 

  • Arnaud-Haond S, Goyard E, Vonau V, Herbaut C, Prou J, Saulnier D (2007) Pearl formation: persistence of the graft during the entire process of biomineralization. Mar Biotechnol 9:113–116

    Article  CAS  PubMed  Google Scholar 

  • Blay C, Sham-Koua M, Vonau V, Tetumu R, Cabral P, Ky CL (2013) Influence of nacre deposition rate on cultured pearl grade and colour in the black lipped pearl oyster Pinctada margaritifera using farmed donor families. Aquacu Int. doi:10.1007/s10499-013-9719-5

    Google Scholar 

  • Cochennec N, Haffner P, Levy P, Saulnier D, Fougerouse A, Langy S (2006) Development of an antiseptic procedure to improve retention rates during the grafting process of black-lip oyster, Pinctada margaritifera. World Aquac 37:56–57

    Google Scholar 

  • Gervis MH, Sims NA (1992) The biology and culture of pearl oysters (Bivalvia: Pteridae). ICLARM Stud Rev 21, ODA (Pub), London

  • Harley P, Harley E (1973) A technique for cutting or accessing nerves and connectives in Aplysia californica. Comp Biochem Physiol 45:389–392

    Article  Google Scholar 

  • Ky CL, Blay C, Sham-Koua M, Vanaa V, Lo C, Cabral P (2013) Family effect on cultured pearl quality in black-lipped pearl oyster Pinctada margaritifera and insights for genetic improvement. Aquat Living Resour 26:133–145

    Article  Google Scholar 

  • Lintilhac J (1987) Black pearls of Tahiti. Royal Tahitian Pearl Book, Papeete, p 53

    Google Scholar 

  • Meng Z, Zhang Q, Li Y, Xing K (1994) A study on anatomy of sites of nucleus insertion and pearl culture in Pinctada nigra (Gould). J Fish Sci China 1:25–30

    Google Scholar 

  • Norton JH, Dashorst M, Lansky TM, Robert J, Mayer RJ (1996) An evaluation of some relaxants for use with pearl oysters. Aquaculture 144:39–52

    Article  CAS  Google Scholar 

  • Norton JH, John SL, Turner I, Mayer RJ, Newnham R (2000) Approaches to improve cultured pearl formation in Pinctada margaritifera through use of relaxation, antiseptic application and incision closure during bead insertion. Aquaculture 184:1–17

    Article  Google Scholar 

  • Pit JH, Southgate PC (2003) Fouling and predation; how do they affect growth and survival of the blacklip pearl oyster, Pinctada margaritifera, during nursery culture? Aquac Int 11:545–555

    Article  Google Scholar 

  • Pouvreau S, Gangnery A, Tiapari J, Lagarde F, Garnier M, Bodoy A (2000) Gametogenic cycle and reproductive effort of the tropical blacklip pearl oyster, Pinctada margaritifera (Bivalvia: Pteriidae), cultivated in Takapoto atoll (French Polynesia). Aquat Living Res 13:37–48

    Article  Google Scholar 

  • Sarver D, Sims N (1996) Overcoming the scarcity of pearl oyster (Pinctada margaritifera) in Micronesia and Hawaii: new areas and opportunities for pearl farming. In: Aquaculture 95 Book of Abstracts. World Aquaculture Society, Baton Rouge, LA, USA, 406–407

  • Scoones RJS (1991) Research on practices in the Western Australian cultured pearl industry. Final report to fishing industry research and development council—project BP12, July 1987–June 1990. Broome Pearls Pty Ltd, Perth

  • Sims NA (1992) Abundance and distribution of the black-lip pearl oyster, Pinctada margaritifera (L.), in the Cook Islands, South Pacific. Aust J Mar Freshw Res 43:1409–1421

    Article  Google Scholar 

  • Southgate PC, Beer AC (1997) Hatchery and early nursery culture of the blacklip pearl oyster (Pinctada margaritifera L.). J Shellfish Res 16:561–567

    Google Scholar 

  • Southgate PC, Lucas JS (2008) The pearl oyster. In: Editors Southgate PC, Lucas JS. Elsevier

  • Strake E (2006) Pearls. Rühle-Diebener-verlag GmbH & Co., KG, Germany 706

    Google Scholar 

  • Tayale A, Gueguen Y, Treguier C, Le Grand J, Cochennec-Laureau N, Montagnani C, Ky CL (2012) Evidence of donor effect on cultured pearl quality from a duplicated grafting experiment on Pinctada margaritifera using wild donors. Aquat Living Res 25:269–280

    Article  Google Scholar 

  • Thompson RG, MacDonald BA (1990) The role of environmental conditions in the seasonal synthesis and utilisation of biochemical energy reserves in the giant scallop, Placopecten magellanicus. Can J Fish Aquat Sci 68:750–756

    CAS  Google Scholar 

  • Wada KT (1986) Genetic selection for shell traits in the Japanese pearl oyster, Pinctada fucata martensii. Aquaculture 57:171–176

    Article  Google Scholar 

  • Wada KT (1991) The pearl oyster Pinctada fucata. In: Menzel W (ed) Estuarine and marine bivalve mollusks culture., pp 246–249

    Google Scholar 

  • Wada KT, Komaru A (1996) Color and weight of pearls produced by grafting the mantle tissue from a selected population for white shell color of the Japanese pearl oyster Pinctada fucata martensii (Dunker). Aquaculture 142:25–32

    Article  Google Scholar 

  • Wane G (2013) Les problèmes réels de l'industrie de la perle de Tahiti. Tahiti Pacifique, mensuel d'information et d'économie 260:15–24

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Acknowledgments

We would especially like to thank the host site and employees of Pommier’s Pearl Farm (Arutua atoll-Tuamotu Archipelago-French Polynesia) for the generous support they provided.

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Authors and Affiliations

  1. Ifremer, UMR 241, EIO, Labex Corail, Centre du Pacifique, BP 7004, 98719, Taravao, Tahiti, Polynésie Française

    Chin-Long Ky

  2. UMR729 MISTEA, INRA, Montpellier SupAgro, Service DIM, CHRU de Montpellier, Université Montpellier 1, 2, Place Viala, 34060, Montpellier, France

    Nicolas Molinari

  3. Groupement d’Intérêt Economique Poe O Tahiti Nui, BP 9045, 98715, Papeete, Tahiti, Polynésie Française

    Elisabeth Moe

  4. Ferme perlière POMMIER, BP 3, 98761, Arutua, Archipel des Tuaomotu, Polynésie Française

    Steve Pommier

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  1. Chin-Long Ky
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  2. Nicolas Molinari
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  3. Elisabeth Moe
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  4. Steve Pommier
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Correspondence to Chin-Long Ky.

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Ky, CL., Molinari, N., Moe, E. et al. Impact of season and grafter skill on nucleus retention and pearl oyster mortality rate in Pinctada margaritifera aquaculture. Aquacult Int 22, 1689–1701 (2014). https://doi.org/10.1007/s10499-014-9774-6

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  • DOI: https://doi.org/10.1007/s10499-014-9774-6

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