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The effect of domestication on a brown trout (Salmo trutta m fario) broodstock in Hungary


Molecular markers (PCR–RFLP and microsatellite) were used to investigate the genetic background of the only brown trout (Salmo trutta m fario) broodstock in Hungary which due to the hydrogeography of the country should theoretically belong to the Danubian lineage. PCR–RFLP (mitochondrial DNA control region and lactate dehydrogenase and somatolactin genes) as well as microsatellite (BFRO002, OMM1064, Ssa408uos, SsoSL417, SsoSL438) markers were used to distinguish between Danubian and Atlantic lineages of brown trout in the Lillafüred broodstock. Altogether 435 fish were tagged during the experimental period. Due to mortalities, fin clips were collected from 401 individuals. According to the genetic analysis of the mitochondrial DNA, the Danubian haplotype is present in only one individual (0.2 %) of the broodstock. Analysis of the nuclear markers revealed that alleles characteristic of both the Atlantic and the Danubian lineages are found in the population. However, Atlantic alleles dominate throughout the broodstock which is in Hardy–Weinberg equilibrium according to the investigated markers. Results indicate that the original broodstock that was introduced to the farm following its construction in 1933 was of the Atlantic lineage. Although later fish from a local stream were collected and added to the broodstock, the number of these was limited and they were almost exclusively males. Fish from this farm that are stocked by anglers can have a significant genetic impact on trout populations of natural streams.

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  • Bernatchez L (2001) The evolutionary history of brown trout (Salmo trutta L) inferred from phylogeographic, nested clade, and mismatch analyses of mitochondrial DNA variation. Evolution 55:351–379

    CAS  PubMed  Google Scholar 

  • Bernatchez L, Danzmann RG (1993) Congruence in control-region sequence and restriction-site variation in mitochondrial DNA of Brook Charr (Salvelinus fontinalis Mitchill). Mol Biol Evol 10(5):1002–1014

    CAS  Google Scholar 

  • Bogataj K (2010) Genetic analysis of native brown trout (Salmo trutta) populations in Slovenia. Thesis, University of Ljubljana

  • Cairney M, Taggart JB, Høyheim B (2000) Characterization of microsatellite and minisatellite loci in Atlantic salmon (Salmo salar L.) and cross-species amplification in other salmonids. Mol Ecol 9:2175–2178

    Article  CAS  PubMed  Google Scholar 

  • Ford MJ (1998) Testing models of migration and isolation among populations of Chinook salmon (Onchorynchus tshawytscha). Evolution 52(2):539–557

    Article  Google Scholar 

  • Jug T, Berrebi P, Snoj A (2005) Distribution of non-native trout in Slovenia and their introgression with native trout populations as observed through microsatellite DNA analysis. Biol Conserv 123:381–388

    Article  Google Scholar 

  • Marić S, Sušnik S, Simonović P, Snoj A (2006) Phylogeographic study of brown trout from Serbia, based on mitochondrial DNA control region analysis. Genet Sel Evol 38:411–430

    Article  PubMed Central  PubMed  Google Scholar 

  • Marić S, Simonović P, Razpet A (2010) Genetic characterization of broodstock brown trout from Bled fish-farm, Slovenia. Period Biol 112:145–148

    Google Scholar 

  • McMeel OM, Hoey EM, Ferguson A (2001) Partial nucleotide sequences, and routine typing by polymerase chain reaction–restriction fragment length polymorphism, of the brown trout (Salmo trutta) lactate dehydrogenase, LDH-C1*90 and *100 alleles. Mol Ecol 10:29–34

    Article  CAS  PubMed  Google Scholar 

  • Muhlfeld CC, Kalinowski ST, McMahon TE, Taper ML, Painter S, Leary RF, Allendorf FW (2009) Hybridization rapidly reduces fitness of a native trout in the wild. Biol Let 5:328–331

    Article  Google Scholar 

  • Rexroad CE, Coleman RL, Hershberger WK, Killefer J (2002) Rapid communication: thirty-eight polymorphic microsatellite markers for mapping in rainbow trout. J Anim Sci 80:541–542

    CAS  PubMed  Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning. CSH Laboratory Press, Cold Spring Harbor, NY

    Google Scholar 

  • Shimizu M, Kosaka N, Shimada T, Nagahata T, Iwasaki H, Nagai H, Shiba T, Emi M (2002) Universal fluorescent labeling (UFL) method for automated microsatellite analysis. DNA Res 9:173–178

    Article  CAS  PubMed  Google Scholar 

  • Slettan A, Olsaker I, Lie Ø (1995) Atlantic salmon, Salmo salar, microsatellites at the SSOSL25, SSOSL85, SSOSL311, SSOSL417 loci. Anim Genet 26:281–282

    Article  CAS  PubMed  Google Scholar 

  • Slettan A, Olsaker I, Lie Ø (1996) Polymorphic Atlantic salmon, Salmo salar L., microsatellites at the SSOSL438. SSOSL439 and SSOSL444 loci. Anim Genet 27:57–58

    Article  CAS  PubMed  Google Scholar 

  • Snoj A, Marčeta B, Sušnik S, Melkič E, Meglič V, Dovč P (2002) The taxonomic status of the ‘sea trout’ from the north Adriatic Sea, as revealed by mitochondrial and nuclear DNA analysis. J Biogeogr 29:1179–1185

    Article  Google Scholar 

  • Snoj A, Glamuzina B, Razpet A, Zablocki J, Bogut I, Lerceteau-Köhler E, Pojskić N, Sušnik S (2010) Resolving taxonomic uncertainties using molecular systematics: Salmo dentex and the Balkan trout community. Hydrobiologia 651:199–212

    Article  CAS  Google Scholar 

  • Sušnik S, Snoj A, Pohar J, Dovč P (1997) The microsatellite marker (BFRO 002) characteristic for different geographically remote brown trout, Salmo trutta L., populations. Anim Genet 28:372

    Article  PubMed  Google Scholar 

  • Sušnik S, Snoj A, Dovč P (2001) Evolutionary distinctness of grayling (Thymallus thymallus) inhabiting the Adriatic river system, as based on mtDNA variation. Biol J Linnean Soc 74:375–385

    Google Scholar 

  • Taylor EB (1991) A review of local adaptation in Salmonidae, with particular reference to Pacific and Atlantic salmon. Aquaculture 98:185–207

    Article  Google Scholar 

  • Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) Micro-Checker: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538

    Article  Google Scholar 

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The work was supported by the GOP‐111‐09/1‐2010‐0141 project.

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Correspondence to Ákos Horváth.

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Horváth, Á., Hoitsy, G., Kovács, B. et al. The effect of domestication on a brown trout (Salmo trutta m fario) broodstock in Hungary. Aquacult Int 22, 5–11 (2014).

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  • Brown trout
  • Genetics
  • Stocking
  • Population structure