Abstract
The effect of different dietary concentrations of shrimp protein hydrolysate (SPH) on digestive enzyme activity of Nile tilapia juveniles was evaluated. SPH concentrations in diets were 0, 15, 30 and 60 g kg−1 (treatments SPH0, SPH15, SPH30 and SPH60, respectively). Hemoglobin, azocasein, BApNA (Nα-benzoyl-dl-arginine-p-nitroanilide), SApNA (Suc-Ala-Ala-Pro-Phe p-nitroanilide), aminoacyl of β-naphthylamide and starch were used as substrates for enzyme activity determinations. The activity of total alkaline protease was significantly higher (P < 0.05) in fish under SPH15 and SPH60 treatments than in the control (SPH0). However, the effect was not dose-dependent. Substrate-SDS-PAGE was also performed to evaluate changes in the profile of Nile tilapia digestive proteases caused by SPH. Substrate-SDS-PAGE revealed 12 active proteolytic bands, eight of which responded to SPH dietary incorporation. Inhibition substrate-SDS-PAGE indicated a decrease in the activity of three enzymes, with trypsin activity decreasing with the increase of SPH concentration, whereas the opposite occurred for two aminopeptidases. Distinct protease profiles were also found for each treatment, suggesting adaptability of digestive proteases from Nile tilapia to the different diets.
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Acknowledgments
The authors would like to thank Albérico Espírito Santo and João Virgínio for their technical assistance and Poytara Ltda for the preparation of the diets. This study was supported by the Financiadora de Estudos e Projetos (FINEP/RECARCINE), Ministério de Aquicultura e Pesca—(MAP), Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA), Conselho Nacional de Pesquisa e Desenvolvimento Científico (CNPq), Fundação de Apoio à Ciência e Tecnologia do Estado de Pernambuco (FACEPE), Petróleo do Brasil S/A (PETROBRAS) and the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). D. Lemos received funding from FAPESP (05/50578-2; 07/07051-9) and CNPq/SEAP (504031/03-1; 308444/06-0; 474222/07-1).
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Santos, J.F., Castro, P.F., Leal, A.L.G. et al. Digestive enzyme activity in juvenile Nile tilapia (Oreochromis niloticus, L) submitted to different dietary levels of shrimp protein hydrolysate. Aquacult Int 21, 563–577 (2013). https://doi.org/10.1007/s10499-012-9589-2
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DOI: https://doi.org/10.1007/s10499-012-9589-2