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Aquaculture International

, Volume 21, Issue 4, pp 759–768 | Cite as

Induction of viable gynogenetic progeny using eggs and UV-irradiated sperm from the Chinese tetraploid loach, Misgurnus anguillicaudatus

  • Ya-Juan Li
  • Zhuo Yu
  • Ming-Zhao Zhang
  • Cong Qian
  • Syuiti Abe
  • Katsutoshi Arai
Article

Abstract

When eggs from the Chinese tetraploid loach that had 100 chromosomes were fertilized with UV-irradiated sperm, we obtained viable gynogenetic progeny without any additional treatment for the duplication of maternal chromosomes, which survived beyond first feeding towards adult stage of development. Gynogenetic progeny were determined to be diploid since they possessed 50 chromosomes, along with two chromosomes bearing nucleolar organizing regions (NORs), detected by silver nitrate staining (Ag-NORs), chromomycin-A3 (CMA3)-positive sites and fluorescence in situ hybridization (FISH) signals for rDNA loci. In contrast, when gynogens were induced using eggs from diploid loach fertilized by UV-irradiated sperm, but without chromosome doubling, we found that all resultant progeny were non-viable haploid gynogens with 25 chromosomes, along with one NOR-bearing chromosome detected by Ag-NORs, CMA3 and FISH. These observations demonstrate the true genetic tetraploid nature of the Chinese loach possessing 100 chromosomes, and the potential use of this tetraploid as a source of functional diploid gametes for further ploidy manipulation experiments.

Keywords

Chromosome Gametes FISH Polyploid 

Notes

Acknowledgments

This study was supported in part by Grants-in-Aid for Scientific Research (B) (No. 21380114) from the Japan Society for the Promotion of Science (JSPS) to Katsutoshi Arai and by the Ronpaku (Dissertation Ph.D.) Program (CSC-10610) from JSPS to Ya-Juan Li.

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Ya-Juan Li
    • 1
  • Zhuo Yu
    • 1
  • Ming-Zhao Zhang
    • 1
  • Cong Qian
    • 1
  • Syuiti Abe
    • 2
  • Katsutoshi Arai
    • 2
  1. 1.Life Science and Technology InstituteDalian Ocean UniversityDalianPeople’s Republic of China
  2. 2.Faculty and Graduate School of Fisheries SciencesHokkaido UniversityHakodate, HokkaidoJapan

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